Tested Applications
| Positive WB detected in | HeLa cells, MDA-MB-231 cells, SW480 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30058-1-AP targets ULBP3 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32424 Product name: Recombinant human ULBP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Sequence: DAHSLWYNFTIIHLPRHGQQWCEVQSQVDQKNFLSYDCGSDKVLSMGHLEEQLYATDAWGKQLEMLREVGQRLRLELADTELEDFTPSGPLTLQVRMSCECEADGYIRGSWQFSFDGRKFL Predict reactive species |
| Full Name | UL16 binding protein 3 |
| Calculated Molecular Weight | 28 kDa |
| Observed Molecular Weight | 28 kDa |
| Gene Symbol | ULBP3 |
| Gene ID (NCBI) | 79465 |
| RRID | AB_3086222 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BZM4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ULBP3 (UL16 binding protein-3), a class 1 major histocompatibility complex-like (MHC-like) molecule and member of the family of ligands of the killer cell lectin-like receptor subfamily K member 1 (NKG2D) (PMID:31292299). ULBP3 are expressed at the cell surface of multiple types of cells and tissues as a glycosylphosphatidylinositol (GPI)-linked proteins (PMID:22741585).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ULBP3 antibody 30058-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ULBP3 Polyclonal antibody (catalog number 30058-1-AP) has a total of 3 citations. These appear in EMBO Molecular Medicine, Cell Oncology, and Cell Reports Medicine. The associated research fields include natural killer (NK) cell-driven anti-tumor immunity, TGF-β1 suppression, immunotherapy in triple-negative breast cancer, and NKG2D ligand-mediated CAR-T cell costimulation.
| Species | Application | Title |
|---|---|---|
EMBO Mol Med Loss of EHMT2 enhances NK cell-driven anti-tumor immunity through TGF-β1 suppression.
| ||
Cell Oncol (Dordr) TAK-981 potentiates doxorubicin immunocide in triple-negative breast cancer by IFN I-dependent NK cell stimulation. | ||
Cell Rep Med NKG2D overexpression potentiates CAR-T cells through costimulation by tumor NKG2D ligands. |
