AKAP9 Polyclonal antibody

AKAP9 Polyclonal Antibody for WB, ELISA

Cat No. 30290-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

A kinase anchor protein 9, AKAP 120 like protein, AKAP 350, AKAP 450, AKAP 9

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis using 30290-1-AP
Various lysates were subjected to Tris-Acetate gel system followed by western blot with 30290-1-AP (AKAP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 30290-1-AP
Various lysates were subjected to Tris-Acetate gel system followed by western blot with 30290-1-AP (AKAP9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

K-562 cells, U2OS cells, C2C12 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30290-1-AP targets AKAP9 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag32425 Product name: Recombinant human AKAP9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-152 aa of NM_005751.4 Sequence: MEDEERQKKLEAGKAKLAQFRQRKAQSDGQSPSKKQKKKRKTSSSKHDVSAHHDLNIDQSQCNEMYINSSQRVESTVIPESTIMRTLHSGEITSHEQGFSVELESEISTTADDCSSEVNGCSFVMRTGKPTNLLREEEFGVDDSYSEQGAQD Predict reactive species
Full Name	A kinase (PRKA) anchor protein (yotiao) 9
Calculated Molecular Weight	453 kDa
Observed Molecular Weight	450-500 kDa
GenBank Accession Number	NM_005751.4
Gene Symbol	AKAP9
Gene ID (NCBI)	10142
RRID	AB_3086287
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q99996
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AKAP9 antibody 30290-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The AKAP9 Polyclonal antibody (Cat# 30290-1-AP) has 1 total citation. It was cited in Frontiers in Immunology (Impact Factor 7), in a 2023 study by Chuanqing Jing et al. The research focuses on cuproptosis gene signatures and their prognostic and immunological features in idiopathic pulmonary fibrosis, with the antibody applied in Western Blot (WB) on human samples.

Species	Application	Title
human	WB	Front Immunol A comprehensive cuproptosis score and associated gene signatures reveal prognostic and immunological features of idiopathic pulmonary fibrosis Authors - Chuanqing Jing View Article