Tested Applications
| Positive WB detected in | HEK-293T cells, HuH-7 cells, MDA-MB-453 cells, mouse brain tissue |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30508-1-AP targets ARHGEF11 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag33104 Product name: Recombinant human ARHGEF11 protein Source: e coli.-derived, PGEX-4T Tag: GST Sequence: ETQAAQEPEDDLTPTPSVISVTSHPWDPGSPGQAPPGGEGDNTQLAGLEGERPEQEDMGLCSLEHLPPRTRNSGIWESPELDRNLAEDASSTEAAGGYKVVRKAEVAGSKVVPALPESGQSEPGPPEVEGGTKATGNCFYVSMPSGPPDSSTDHSEAPMSPPQPDSLPAGQTEPQPQLQGGNDDPRRPSRSPPSLALRDVGMIFHTIEQLTLKLNRLKDMELAHRELLKSLGGESSGGTTPVGSFHTEAARWTDGSLSPPAKEPLASDSRNSHELGPCPEDGSDAPLEDSTADAAASPGP Predict reactive species |
| Full Name | Rho guanine nucleotide exchange factor (GEF) 11 |
| Calculated Molecular Weight | 168 kDa |
| Observed Molecular Weight | 200-220 kDa |
| Gene Symbol | ARHGEF11 |
| Gene ID (NCBI) | 9826 |
| RRID | AB_3086341 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15085 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ARHGEF11 (Rho guanine nucleotide exchange factor 11) is a specific guanine nucleotide exchange factor (GEF) for RhoA, which is a critical regulator of actin cytoskeleton dynamics and organization of dendritic spines and inhibitor of spine maintenance (PMID: 28036092). ARHGEF11 has been proven to promote tumor metastasis in glioblastoma and ovarian carcinoma (PMID: 33122451).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ARHGEF11 antibody 30508-1-AP | Download protocol |
| WB protocol for ARHGEF11 antibody 30508-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot application. The user reported that the antibody worked well at a 1:2000 dilution, noting that the uppermost bands correspond to the PDZ domain. The experiment was performed on MSCs (mesenchymal stem cells). Overall, the feedback is positive, indicating reliable performance and clear band recognition at the recommended dilution.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Udesh (Verified Customer) (08-19-2024) | Worked well at 1:2000 - uppermost bands are PDZ
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