Tested Applications
| Positive WB detected in | DU 145 cells, HEK-293T cells |
| Positive IP detected in | DU 145 cells |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30834-1-AP targets ZFC3H1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34224 Product name: Recombinant human ZFC3H1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1800-1989 aa of BC156732 Sequence: RNKVQEFKFFTDLVNRCLVTVPARYPIPFSSADYWSNYEFHNRVIFFYLSCVPKTQHSKTLERFCSVMPANSGLALRLLQHEWEESNVQILKLQAKMFTYNIPTCLATWKIAIAAEIVLKGQREVHRLYQRALQKLPLCASLWKDQLLFEASEGGKTDNLRKLVSKCQEIGVSLNELLNLNSNKTESKNH Predict reactive species |
| Full Name | zinc finger, C3H1-type containing |
| Calculated Molecular Weight | 226 kDa |
| Observed Molecular Weight | 250 kDa |
| GenBank Accession Number | BC156732 |
| Gene Symbol | ZFC3H1 |
| Gene ID (NCBI) | 196441 |
| RRID | AB_3669762 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60293 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZFC3H1, also known as CCDC131 and PSRC2, is a large protein (1989 amino acids), with a C3H1- type zinc finger motif in its center, and contains five tetratricopeptide (TRP) repeats and six halftetratricopeptide (HAT) repeats at the C-terminal region. It is involved in the processing of a variety of RNAs and plays a critical role in the degradation of nuclear RNAs (PMID: 34514952).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ZFC3H1 antibody 30834-1-AP | Download protocol |
| IP protocol for ZFC3H1 antibody 30834-1-AP | Download protocol |
| WB protocol for ZFC3H1 antibody 30834-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The product received a 5-star rating for its performance in immunoprecipitation. The reviewer used it on Jurkat cells with 3 µg of antibody per 250 µg of nuclear extracts and reported excellent results. Overall, the feedback highlights the antibody's strong and reliable performance in IP applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Fabienne (Verified Customer) (09-12-2025) | Works great in immunoprecipitation (used 3µg antibody per 250µg nuclear extracts)
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