Tested Applications
| Positive WB detected in | HeLa cells, A549 cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30923-1-AP targets AHCTF1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32788 Product name: Recombinant human AHCTF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2067-2266 aa of NM_001323342.1 Sequence: ERTDEMTHKETNEQEERLLATASFTKSSRSSRTRSSKAILLPDLSEPNNEPLFSPASEVPRKAKAKKIEVPAQLKELVSDLSSQFVISPPALRSRQKNTSNKNKLEDELKDDAQSVETLGKPKAKRIRTSKTKQASKNTEKESAWSPPPIEIRLISPLASPADGVKSKPRKTTEVTGTGLGRNRKKLSSYPKQILRRKML Predict reactive species |
| Full Name | AT hook containing transcription factor 1 |
| Calculated Molecular Weight | 252 kDa |
| Observed Molecular Weight | 270 kDa |
| GenBank Accession Number | NM_001323342.1 |
| Gene Symbol | AHCTF1 |
| Gene ID (NCBI) | 25909 |
| RRID | AB_3669786 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WYP5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AHCTF1, also known as ELYS, TMBS62, is a large multifunctional protein. It plays essential roles in nuclear pore assembly and mitosis. AHCTF1 was expressed at higher levels in embryonic liver, spleen and thymus compared to the corresponding adult tissues (PMID: 11952839) and has 3 isoforms with MW of 252~256 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AHCTF1 antibody 30923-1-AP | Download protocol |
| WB protocol for AHCTF1 antibody 30923-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AHCTF1 Polyclonal antibody (Cat# 30923-1-AP) has 3 citations published in Cell Death Discovery, Cell Bioscience, and Digestive Diseases and Sciences. The associated research fields include nuclear RNA export mechanisms (exon junction complex and nuclear pore complex), hepatocellular carcinoma stemness driven by Hedgehog signaling, and tumor progression in hepatocellular carcinoma. All cited applications were Western Blot in human samples.
| Species | Application | Title |
|---|---|---|
Cell Death Discov Exon junction complex and nuclear pore complex components preferentially govern the nuclear export of spliced lncRNAs. | ||
Cell Biosci ELYS promotes hepatocellular carcinoma stemness by activating a FOXO6-NUP205 transcriptional module downstream of PI3K/AKT to drive Hedgehog signaling. | ||
Dig Dis Sci AHCTF1 Functions as an Oncogenic Factor and Promotes Tumor Progression in Hepatocellular Carcinoma
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