Tested Applications
| Positive WB detected in | mouse testis tissue, A549 cells, mouse kindey tissue, mouse liver tissue |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30941-1-AP targets RNF180 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34138 Product name: Recombinant human RNF180 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 220-416 aa of BC101277 Sequence: ATRAFHRKSHSLDLNISEKLTLLPTLYEIHSKTTAYSRLNETQPIDLSGLPLQSSKNSYSFQNPSSFDPSMLLQRFSVAPHETQTQRGGEFQCGLEAASVYSDHTNTNNLTFLMDLPSAGRSMPEASDQEEHLSPLDFLHSANFSLGSINQRLNKRERSKLKNLRRKQRRRERWLQKQGKYSGVGLLDHMVSIYLLI Predict reactive species |
| Full Name | ring finger protein 180 |
| Calculated Molecular Weight | 592 aa, 68 kDa |
| Observed Molecular Weight | 70 kDa,48kDa |
| GenBank Accession Number | BC101277 |
| Gene Symbol | RNF180 |
| Gene ID (NCBI) | 285671 |
| RRID | AB_3669788 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q86T96 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RNF180 antibody 30941-1-AP | Download protocol |
| IHC protocol for RNF180 antibody 30941-1-AP | Download protocol |
| WB protocol for RNF180 antibody 30941-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RNF180 Polyclonal antibody (Cat# 30941-1-AP) has 2 total citations. These appear in Scientific Reports and iScience. Both studies fall within the oncology/cancer biology field: one investigates RNF180's role in modulating the EGFR/PI3K/AKT pathway to reduce cisplatin resistance in non-small cell lung cancer, while the other examines how RNF180 loss stabilizes BCAS4 to enhance PD-L1 expression and immune evasion in hepatocellular carcinoma.
| Species | Application | Title |
|---|---|---|
Sci Rep The E3 ubiquitin ligase RNF180 modulates the EGFR/PI3K/AKT pathway to reduce cisplatin resistance in non-small cell lung cancer. | ||
iScience RNF180 loss stabilizes BCAS4 to enhance PD-L1 expression and immune evasion in hepatocellular carcinoma. |
Reviews
What customers say
The product has received one review with a 5-star rating. The reviewer used the antibody for Western Blot at a 1:1000 dilution on A549 cells and reported that it performed very well, producing clear and reliable results. Overall, the feedback is positive, indicating strong performance in its tested application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Edma (Verified Customer) (03-03-2025) | Antibody worked very well in western blot
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