Tested Applications
| Positive WB detected in | mouse liver tissue, mouse lung tissue, mouse spleen tissue |
| Positive IHC detected in | mouse spleen tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue, mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31024-1-AP targets CD163 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | CD163 antigen |
| Calculated Molecular Weight | 121 kDa |
| Observed Molecular Weight | 160 kDa |
| GenBank Accession Number | NM_053094.2 |
| Gene Symbol | Cd163 |
| Gene ID (NCBI) | 93671 |
| RRID | AB_3669819 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q2VLH6-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD163 antibody 31024-1-AP | Download protocol |
| IHC protocol for CD163 antibody 31024-1-AP | Download protocol |
| WB protocol for CD163 antibody 31024-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD163 Polyclonal antibody (Cat# 31024-1-AP) has 5 citations published in MedComm, Biomaterials, Oncoimmunology, Experimental Cell Research, and Journal of Leukocyte Biology. Research fields span glioblastoma vascular biology, wound regeneration with macrophage reprogramming, pancreatic cancer immunotherapy, prostate cancer macrophage M2 polarization, and colorectal cancer macrophage regulation. Applications cited include WB, IHC, and IF in mouse models.
| Species | Application | Title |
|---|---|---|
MedComm (2020) Phase Separation-Mediated SRF/P54nrb Transcription Complex Shapes the Vasculature Microenvironment via Upregulating OLFML3 in Glioblastoma. | ||
Biomaterials Molybdoenzymes-emulating bio-heterojunction hydrogel with rapid disinfection and macrophage reprogramming for wound regeneration | ||
Oncoimmunology Mild microwave hyperthermia promotes mitotic catastrophe, induces time-delayed cGAS-STING activation and restores sensitivity to anti-PDL1 therapy in Pan02 pancreatic cancer model. | ||
Exp Cell Res TFAP4/DLGAP5 promotes tumor progression and macrophage M2 polarization in prostate cancer by activating the JAK2/STAT3 signaling. | ||
J Leukoc Biol TIPE regulates TGFB2 expression and induces extracellular M2 polarization in CRC |
