Tested Applications
| Positive WB detected in | HCT 116 cells, HT-1080 cells |
| Positive IHC detected in | human colon cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31106-1-AP targets KIAA1199 / CEMIP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34315 Product name: Recombinant human KIAA1199 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 180-420 aa of BC020256 Sequence: FERSWGHRGVIVHVIDPKSGTVIHSDRFDTYRSKKESERLVQYLNAVPDGRILSVAVNDEGSRNLDDMARKAMTKLGSKHFLHLGFRHPWSFLTVKGNPSSSVEDHIEYHGHRGSAAARVFKLFQTEHGEYFNVSLSSEWVQDVEWTEWFDHDKVSQTKGGEKISDLWKAHPGKICNRPIDIQATTMDGVNLSTEVVYKKGQDYRFACYDRGRACRSYRVRFLCGKPVRPKLTVTIDTNVN Predict reactive species |
| Full Name | KIAA1199 / CEMIP |
| Calculated Molecular Weight | 1361 aa, 153 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC020256 |
| Gene Symbol | KIAA1199 |
| Gene ID (NCBI) | 57214 |
| RRID | AB_3669858 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8WUJ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KIAA1199, also known as CEMIP, was first discovered as an inner-ear protein whose genetic mutations were linked to nonsyndromic hearing loss. KIAA1199 is involved in hyaluronan degradation. Studies have shown that KIAA1199 is overexpressed in many types of cancers, suggesting a critical role of KIAA1199 in cancer progression.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KIAA1199 / CEMIP antibody 31106-1-AP | Download protocol |
| IHC protocol for KIAA1199 / CEMIP antibody 31106-1-AP | Download protocol |
| WB protocol for KIAA1199 / CEMIP antibody 31106-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KIAA1199/CEMIP polyclonal antibody (Cat# 31106-1-AP) has 2 published citations. These appear in BBA Advances and Frontiers in Bioscience-Landmark Edition. The research fields covered include lung cancer (specifically gefitinib resistance in EGFR-mutant lung cancer) and colorectal cancer (single-cell analysis of Lynch syndrome progression). Both studies used human samples, with applications including WB, IHC, and IF.
| Species | Application | Title |
|---|---|---|
BBA Adv CEMIP promotes gefitinib resistance in lung cancer with EGFR-mutation possibly through forming a stable complex CEMIP/c-MYC.
| ||
Front Biosci (Landmark Ed) From Normal Mucosa to Colorectal Cancer in Lynch Syndrome: Single-Cell Dissection of Cellular Heterogeneity and Communication Networks. |
