Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31175-1-AP targets HNRNPAB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35010 Product name: Recombinant human HNRNPAB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-62 aa of BC036708 Sequence: MSEAGEEQPMETTGATENGHEAVPEGESPAGAGTGAAAGAGGATAAPPSGNQNGAEGDQINA Predict reactive species |
| Full Name | heterogeneous nuclear ribonucleoprotein A/B |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36-40 kDa |
| GenBank Accession Number | BC036708 |
| Gene Symbol | HNRNPAB |
| Gene ID (NCBI) | 3182 |
| RRID | AB_3669882 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q99729 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Heterogeneous nuclear ribonucleoprotein AB (HNRNPAB) is a member of the hnRNP family involved in multiple forms of transcriptional control. It plays an important role in normal biological processes and tumor development and progression. HNRNPAB acts as a direct transcriptional target of c-Myc and promotes G1/S cell cycle progression and cell proliferation via CDK4(PMID: 36657708).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HNRNPAB antibody 31175-1-AP | Download protocol |
| IHC protocol for HNRNPAB antibody 31175-1-AP | Download protocol |
| WB protocol for HNRNPAB antibody 31175-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HNRNPAB Polyclonal antibody (Cat# 31175-1-AP) has 1 total citation. It appears in bioRxiv, in a study on proteomic screening identifying HNRNPA2B1 as an epigenetic repressor of Epstein-Barr virus reactivation. The research field encompasses virology, epigenetics, and protein-proteomics. The antibody was used for Western blot (WB) in human samples.
| Species | Application | Title |
|---|---|---|
bioRxiv Proteomic Screening Identifies HNRNPA2B1 as an Epigenetic Repressor of Epstein-Barr Virus Reactivation. |
