Tested Applications
| Positive WB detected in | human placenta tissue, mouse skeletal muscle tissue, rat heart tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31181-1-AP targets LAMA2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34494 Product name: Recombinant human LAMA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2100-2250 aa of NM_000426 Sequence: IDKLKPIKELEDNLKKNISEIKELINQARKQANSIKVSVSSGGDCIRTYKPEIKKGSYNNIVVNVKTAVADNLLFYLGSAKFIDFLAIEMRKGKVSFLWDVGSGVGRVEYPDLTIDDSYWYRIVASRTGRNGTISVRALDG Predict reactive species |
| Full Name | laminin, alpha 2 |
| Calculated Molecular Weight | 344 kDa |
| Observed Molecular Weight | 344 kDa |
| GenBank Accession Number | NM_000426 |
| Gene Symbol | LAMA2 |
| Gene ID (NCBI) | 3908 |
| RRID | AB_3669885 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P24043 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The laminin subunit alpha 2 (LAMA2) gene encodes an alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Basal laminae are structural components of the extracellular matrix that influence cell proliferation and differentiation,7 and laminin subunit alpha 2 (LAMA2) is the major component of the basal laminae-α subunit of laminin. (PMID: 30728939)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LAMA2 antibody 31181-1-AP | Download protocol |
| IHC protocol for LAMA2 antibody 31181-1-AP | Download protocol |
| WB protocol for LAMA2 antibody 31181-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this LAMA2 antibody (used at 1:100 for immunofluorescence on mouse skeletal muscle) is highly favorable, rated 5 stars. The reviewer, after trying several LAMA2 antibodies, found this primary antibody performed very well on mouse skeletal muscle tissue, enabling them to progress with their PhD thesis. Overall, the feedback highlights strong staining performance and reliability for immunofluorescence applications in mouse skeletal muscle.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Diogo (Verified Customer) (10-23-2025) | I have tried several LAMA2 antibodies and this primary antibody worked very well with mouse skeletal muscle ,which allowed to progress on my PhD thesis.
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