Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells |
| Positive IP detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31300-1-AP targets ZNF638 in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35296 Product name: Recombinant human ZNF638 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1777-1978 aa of NM_014497.4 Sequence: VDEVGEEEDGDNDLKVELAQSKNDHPTDKKGNRKKRAVDTKKTKLESLSQVGPVNENVMEEDLKTMIERHLTAKTPTKRVRIGKTLPSEKAVVTEPAKGEEAFQMSEVDEESGLKDSEPERKRKKTEDSSSGKSVASDVPEELDFLVPKAGFFCPICSLFYSGEKAMTNHCKSTRHKQNTEKFMAKQRKEKEQNEAEERSSR Predict reactive species |
| Full Name | zinc finger protein 638 |
| Calculated Molecular Weight | 221kDa |
| Observed Molecular Weight | 270-300 kDa |
| GenBank Accession Number | NM_014497.4 |
| Gene Symbol | ZNF638 |
| Gene ID (NCBI) | 27332 |
| RRID | AB_3669937 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q14966 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZNF638 (Zinc finger protein 638) is also named as NP220 and ZFML. ZNF638 is a newly discovered member of zinc finger protein family for adipocyte function in vitro. And ZNF638regulates triglyceride metabolism via ANGPTL8 in an estrogen dependent manner (PMID: 38211696). ZNF638 plays a role as a transcriptional coregulator of adipocyte differentiation via induction of PPARγ in cooperation with CCAAT/enhancer binding proteins (C/EBPs) (PMID: 25024404). ZNF638 is localized in nuclear bodies enriched with splicing factors (PMID: 25024404). The ZNF638 protein is modified and exhibits a molecular weight of ~300 kDa (PMID: 33040080)
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ZNF638 antibody 31300-1-AP | Download protocol |
| WB protocol for ZNF638 antibody 31300-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZNF638 Polyclonal antibody (Cat# 31300-1-AP) has 1 citation to date. It was cited in Journal of Translational Medicine (IF 9.7), in a study on an integrative epigenomic and single-cell framework identifying a prognostic DNA methylation risk score and ZNF638 silencing in urothelial carcinoma. The associated research fields include epigenetics, single-cell genomics, and urothelial carcinoma (cancer biology).
| Species | Application | Title |
|---|---|---|
J Transl Med An integrative epigenomic and single-cell framework identifies a prognostic DNA methylation risk score and ZNF638 silencing in urothelial carcinoma. |
Reviews
What customers say
Customers used this antibody for Western Blot at dilutions ranging from 1:2000 to 1:5000 across HEK293T, RKO, U2OS, and mESC cell lines. Overall ratings averaged around 4 out of 5. Reviewers generally detected a band in the expected ~221 kDa region, though several noted additional non-specific bands, particularly in the 100–250 kDa range. One user described it as a "great antibody," while others acknowledged some background signal but still found the target band recognizable. No major issues with specificity or sensitivity were reported.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Moritz (Verified Customer) (09-26-2025) | Several band are visible in the area between 150-250 kDa
 |
Aleksandr (Verified Customer) (07-29-2025) | Antibody recognise a band of the expected size with some other prominent, likely non-specific ones
 |
Tsimafei (Verified Customer) (08-07-2024) | Great antibody
 |
Julia (Verified Customer) (08-07-2024) | Band below 250 kDa marker is likely the Znf639 (~221kDa), there is also strong signal between 100-50kDa. I used 30ug of mESC protein extract.
 |
