Tested Applications
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | rat testis tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31344-1-AP targets CFAP70 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35209 Product name: Recombinant human TTC18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-200 aa of BC032856 Sequence: MEQVPSAGRLVQITVTEGYDLKGFKGDTPVTFIRAEFNQVVLGDSAKITVSPEGSAKYNFTSSFEFNPEGGITSDDLAHKPVFLTVTEVLPKEKKQKEEKTLILGQAVVDLLPLLEGQSSFQTTVPLHPVQGSPLETPRSSAKQCSLEVKVLVAEPLLTTAQISGGNLLKVTLEAAYSVPESFIPTGPGQNYMVGLQVPS Predict reactive species |
| Full Name | tetratricopeptide repeat domain 18 |
| Calculated Molecular Weight | 126 kDa |
| Observed Molecular Weight | 126 kDa |
| GenBank Accession Number | BC032856 |
| Gene Symbol | TTC18 |
| Gene ID (NCBI) | 118491 |
| RRID | AB_3669954 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5T0N1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cilia- and flagella-associated protein 70 (CFAP70) harbours eight tetratricopeptide repeats (TPRs) and bound tightly to the ciliary axoneme. CFAP70 is necessary to assemble spermatid flagella and could be used as a diagnostic target for male infertility with OAT in the clinic.CFAP70 is a novel regulatory component of the ODA in motile cilia and flagella, and that the N-terminus is important for its ciliary localization (PMID: 30158508).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CFAP70 antibody 31344-1-AP | Download protocol |
| IHC protocol for CFAP70 antibody 31344-1-AP | Download protocol |
| IP protocol for CFAP70 antibody 31344-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CFAP70 Polyclonal antibody (Cat# 31344-1-AP) has a total of 2 citations. These appear in Ecotoxicology and Environmental Safety and Asian Journal of Andrology. The associated research fields include reproductive toxicology (PFOA exposure effects on sperm head shaping in mice) and clinical andrology/genetics (DNAH17 variants causing sperm flagella morphological abnormalities and male infertility in humans). Applications cited are WB and IF.
| Species | Application | Title |
|---|---|---|
Ecotoxicol Environ Saf PFOA exposure decreases acrosome reaction and increases DNA oxidative damage by impairing the sperm head shaping in mice. | ||
Asian J Androl Novel homozygous or compound heterozygous DNAH17 variants lead to male infertility characterized by multiple morphological abnormalities of the sperm flagella and asthenoteratozoospermia in humans. |
