Tested Applications
| Positive WB detected in | HT-29 cells, HeLa cells, mouse brain tissue, MDA-MB-231 cells, rat brain tissue |
| Positive IHC detected in | human stomach cancer tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31676-1-AP targets SPTAN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36472 Product name: Recombinant human SPTAN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2315-2472 aa of NM_003127.3 Sequence: RNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN Predict reactive species |
| Full Name | spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) |
| Calculated Molecular Weight | 285kDa，2472aa |
| Observed Molecular Weight | 285 kDa,150 kDa |
| GenBank Accession Number | NM_003127.3 |
| Gene Symbol | SPTAN1 |
| Gene ID (NCBI) | 6709 |
| RRID | AB_3670071 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q13813 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SPTAN1 (Nonerythroid spectrin αII), also termed α-Fodrin, is a cytoskeletal protein that belongs to the family of spectrins. Spectrins includes several structural proteins (α- and β-spectrin, α-actinin, dystrophin, and utrophin) that build and stabilize the cytoskeleton by forming a hexagonal mesh under the plasma membrane and ensuring stability and organization of organelles in the cell. SPTAN1 encodes for a 2,472 amino acid protein with a predicted molecular weight of 284 kDa and can be cleaved by calpain leading to a 150 kDa fragment (PMID: 31186638).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SPTAN1 antibody 31676-1-AP | Download protocol |
| IHC protocol for SPTAN1 antibody 31676-1-AP | Download protocol |
| WB protocol for SPTAN1 antibody 31676-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
I'll search for citations for this antibody.31676-1-AP (SPTAN1 Polyclonal Antibody) has 2 total citations. It appears in Advanced Science (Adv Sci, IF 14.1) for an immunofluorescence (IF) study on supramolecular complexes in chronic neuropathic pain (mouse), and in Journal of Integrative Neuroscience (IF 3.3) for a Western blot (WB) study of an acupuncture-modulated NMDAR pathway in spastic hemiplegia following ischemic stroke (rat). Research fields covered include neuropathic pain mechanisms and stroke-related neural signaling.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Targeting Supramolecular Active Complexes of Na(v)1.7/Na(v)1.8 to Relieve Chronic Neuropathic Pain. | ||
J Integr Neurosci Acupuncture Modulates NMDAR-PP1/Calpain1-KCC2 Pathway to Ameliorate Spinal Hyperexcitability and Spastic Hemiplegia Induced by Ischemic Stroke. |
