CDK5RAP2 Polyclonal antibody

CDK5RAP2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 31702-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

C48, CDK5 activator-binding protein C48, CDK5 regulatory subunit-associated protein 2, Centrosome-associated protein 215, CEP215

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIHC

WB analysis using 31702-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 31702-1-AP (CDK5RAP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 31702-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 31702-1-AP (CDK5RAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse liver using 31702-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31702-1-AP (CDK5RAP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 31702-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31702-1-AP (CDK5RAP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human rectal cancer using 31702-1-AP
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 31702-1-AP (CDK5RAP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse colon using 31702-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31702-1-AP (CDK5RAP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 31702-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31702-1-AP (CDK5RAP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat kidney using 31702-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 31702-1-AP (CDK5RAP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat liver using 31702-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31702-1-AP (CDK5RAP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, A549 cells
Positive IHC detected in	rat kidney tissue, mouse liver tissue, human placenta tissue, human rectal cancer tissue, mouse colon tissue, mouse kidney tissue, rat liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31702-1-AP targets CDK5RAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag36245 Product name: Recombinant human CDK5RAP2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1221-1571 aa of BC146782 Sequence: MQLFSEIHNLQNKFRDLSPPRYDSLVQSQARELSLQRQQIKDGHGICVISRQHMNTMIKAFEELLQASDVDYCVAEGFQEQLNQCAELLEKLEKLFLNGKSVGVEMNTQNELMERIEEDNLTYQHLLPESPEPSASHALSDYETSEKSFFSRDQKQDNETEKTSVMVNSFSQDLLMEHIQEIRTLRKRLEESIKTNEKLRKQLERQGSEFVQGSTSIFASGSELHSSLTSEIHFLRKQNQALNAMLIKGSRDKQKENDKLRESLSRKTVSLEHLQREYASVKEENERLQKEGSEKERHNQQLIQEVRCSGQELSRVQEELKLRQQLLSQNDKLLQSLRVELKAYEKLDEEH Predict reactive species
Full Name	CDK5 regulatory subunit associated protein 2
Calculated Molecular Weight	1893 aa, 215 kDa
Observed Molecular Weight	210-215 kDa
GenBank Accession Number	BC146782
Gene Symbol	CDK5RAP2
Gene ID (NCBI)	55755
RRID	AB_3670087
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity Purification
UNIPROT ID	Q96SN8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin-dependent kinase 5 regulatory subunit associated protein 2 (CDK5RAP2) is a pericentriolar matrix (PCM) protein that regulates centriole engagement and centrosome cohesion during the cell cycle. CDK5RAP2 is associated with several cancers, including breast cancer, oral squamous cell carcinoma and haematological malignancies.

Protocols

Product Specific Protocols
IHC protocol for CDK5RAP2 antibody 31702-1-AP	Download protocol
WB protocol for CDK5RAP2 antibody 31702-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
mouse	WB	Adv Sci (Weinh) m6A Reader hnRNPA2B1 Modulates Late Pachytene Progression in Male Meiosis Through Post-Transcriptional Control Authors - Lisha Yin View Article
mouse,human	WB	Cell Death Dis Cdk1-phosphorylated Nur77 accumulates at the centrosome during mitosis to regulate the Cep192-PLK1 signaling axis. Authors - Guobin Xie View Article