Tested Applications
| Positive WB detected in | HeLa cells, A549 cells |
| Positive IHC detected in | rat kidney tissue, mouse liver tissue, human placenta tissue, mouse colon tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31702-1-AP targets CDK5RAP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36245 Product name: Recombinant human CDK5RAP2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1221-1571 aa of BC146782 Sequence: MQLFSEIHNLQNKFRDLSPPRYDSLVQSQARELSLQRQQIKDGHGICVISRQHMNTMIKAFEELLQASDVDYCVAEGFQEQLNQCAELLEKLEKLFLNGKSVGVEMNTQNELMERIEEDNLTYQHLLPESPEPSASHALSDYETSEKSFFSRDQKQDNETEKTSVMVNSFSQDLLMEHIQEIRTLRKRLEESIKTNEKLRKQLERQGSEFVQGSTSIFASGSELHSSLTSEIHFLRKQNQALNAMLIKGSRDKQKENDKLRESLSRKTVSLEHLQREYASVKEENERLQKEGSEKERHNQQLIQEVRCSGQELSRVQEELKLRQQLLSQNDKLLQSLRVELKAYEKLDEEH Predict reactive species |
| Full Name | CDK5 regulatory subunit associated protein 2 |
| Calculated Molecular Weight | 1893 aa, 215 kDa |
| Observed Molecular Weight | 210-215 kDa |
| GenBank Accession Number | BC146782 |
| Gene Symbol | CDK5RAP2 |
| Gene ID (NCBI) | 55755 |
| RRID | AB_3670087 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q96SN8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cyclin-dependent kinase 5 regulatory subunit associated protein 2 (CDK5RAP2) is a pericentriolar matrix (PCM) protein that regulates centriole engagement and centrosome cohesion during the cell cycle. CDK5RAP2 is associated with several cancers, including breast cancer, oral squamous cell carcinoma and haematological malignancies.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CDK5RAP2 antibody 31702-1-AP | Download protocol |
| IHC protocol for CDK5RAP2 antibody 31702-1-AP | Download protocol |
| WB protocol for CDK5RAP2 antibody 31702-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
I'll search for citations for this antibody.CDK5RAP2 Polyclonal antibody (31702-1-AP) has a total of 2 citations.
The publications appear in Advanced Science (Weinheim) (2025, IF 14.1) and Cell Death & Disease (2026, IF 12.2).
Research fields associated with the citations include cell biology and meiosis — specifically m6A reader-mediated post-transcriptional regulation during male meiosis, and cell cycle/mitosis — involving Cdk1-phosphorylated Nur77 at the centrosome regulating the Cep192-PLK1 signaling axis. Both studies used the antibody in Western blot applications across mouse and human samples.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) m6A Reader hnRNPA2B1 Modulates Late Pachytene Progression in Male Meiosis Through Post-Transcriptional Control | ||
Cell Death Dis Cdk1-phosphorylated Nur77 accumulates at the centrosome during mitosis to regulate the Cep192-PLK1 signaling axis. |
