LDAH Polyclonal antibody

LDAH Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 31714-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C2orf43, HLDAH, Lipid Droplet Associated Hydrolase, UPF0554 protein C2orf43

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHCIF/ICC

WB analysis using 31714-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 31714-1-AP (C2orf43 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 31714-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31714-1-AP (LDAH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 31714-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using LDAH antibody (31714-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Protocols

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Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells
Positive IHC detected in	human ovary cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	oleic acid treated HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31714-1-AP targets LDAH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag36372 Product name: Recombinant human C2orf43 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 100-325 aa of BC017473 Sequence: DSNAQEIKDIYGLNGQIEHKLAFLRTHVPKDMKLVLIGHSIGSYFTLQMLKRVPELPVIRAFLLFPTIERMSESPNGRIATPLLCWFRYVLYVTGYLLLKPCPETIKSLLIRRGLQVMNLENEFSPLNILEPFCLANAAYLGGQEMMEVVKRDDETIKEHLCKLTFYYGTIDPWCPKEYYEDIKKDFPEGDIRLCEKNIPHAFITHFNQEMADMIADSLKDDLSKM Predict reactive species
Full Name	chromosome 2 open reading frame 43
Observed Molecular Weight	32 kDa
GenBank Accession Number	BC017473
Gene Symbol	C2orf43
Gene ID (NCBI)	60526
RRID	AB_3670093
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity Purification
UNIPROT ID	Q9H6V9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LDAH, also known as C2orf43, is a newly identified LD protein. It has 4 isoforms and is abundantly expressed in tissues that predominantly store triacylglycerol (TAG), including liver and white and brown adipose tissue (PMID: 24357060).

Protocols

Product Specific Protocols
IF protocol for LDAH antibody 31714-1-AP	Download protocol
IHC protocol for LDAH antibody 31714-1-AP	Download protocol
WB protocol for LDAH antibody 31714-1-AP	Download protocol