Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells, SH-SY5Y cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31833-1-AP targets IK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37095 Product name: Recombinant human IK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC013005 Sequence: MPERDSEPFSNPLAPDGHDVDDPHSFHQSKLTNEDFRKLLMTPRAAPTSAPPSKSRHHEMPREYNEDEDPAARRRKKKSYYAKLRQQEIERERELAEKYRDRAKERRDGVNKDYEETELISTTANYRAVGPTAEADKSAAEKRRQLIQESKFLGGDMEHTHLVKGLDFALLQKVRAEIASKEKEEEELMEKPQKETKKDEDPENKIEFKTRLGRNV Predict reactive species |
| Full Name | IK cytokine, down-regulator of HLA II |
| Calculated Molecular Weight | 557 aa, 66 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC013005 |
| Gene Symbol | IK |
| Gene ID (NCBI) | 3550 |
| RRID | AB_3670123 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q13123 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IK, also known as the nuclear protein RED, is a down-regulator of human leukocyte antigen (HLA) II and interacts with the human SAC protein MAD1, which is required for the localization of the kinetochore of MAD1, for the progression of mitosis and for the activation of the SAC. IK is therefore an important protein for the correct progression of mitosis through the regulation of mitotic kinases and phosphatases. In addition, IK may be involved in pre-mRNA splicing, which may be a useful biomarker for new nuclear structures in cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IK antibody 31833-1-AP | Download protocol |
| IHC protocol for IK antibody 31833-1-AP | Download protocol |
| WB protocol for IK antibody 31833-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IK Polyclonal antibody (Cat# 31833-1-AP) has 1 citation to date. It was cited in Cancer Medicine (2025), in a study on bioinformatics identification of lactate-associated genes in hepatocellular carcinoma, specifically examining G6PD's role in immune modulation. The antibody was applied in IHC on human samples. The research falls within the fields of liver cancer (hepatocellular carcinoma), metabolic gene analysis, and tumor immune microenvironment.
| Species | Application | Title |
|---|---|---|
Cancer Med Bioinformatics Identification of Lactate-Associated Genes in Hepatocellular Carcinoma: G6PD's Role in Immune Modulation |
Reviews
What customers say
The product has received one review with a 5-star rating. The reviewer used the antibody for Western Blot and reported that it works adequately for this application. Overall, the feedback is positive, indicating satisfactory performance in WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sarah (Verified Customer) (10-31-2025) | Antibody works adequately for WB.
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