Tested Applications
| Positive WB detected in | fetal human brain tissue, mouse brain tissue, human heart tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32272-1-AP targets Proenkephalin-A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27972 Product name: Recombinant human PENK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 100-267 aa of BC032505 Sequence: YGGFMKRYGGFMKKMDELYPMEPEEEANGSEILAKRYGGFMKKDAEEDDSLANSSDLLKELLETGDNRERSHHQDGSDNEEEVSKRYGGFMRGLKRSPQLEDEAKELQKRYGGFMRRVGRPEWWMDYQKRYGGFLKRFAEALPSDEEGESYSKEVPEMEKRYGGFMRF Predict reactive species |
| Full Name | proenkephalin |
| Calculated Molecular Weight | 267 aa, 31 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC032505 |
| Gene Symbol | Proenkephalin A |
| Gene ID (NCBI) | 5179 |
| RRID | AB_3742560 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P01210 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Proenkephalin-A (PENK) is also named as Synenkephalin, Met-enkephalin and Opioid growth factor (OGF), and belongs to the opioid neuropeptide precursor family. PENK is a neuropeptide that competes with and mimics the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress (PMID:7057924). PENK hypermethylation is also associated with bladder cancer, hepatocellular carcinoma, colorectal cancer, and prostate cancer (PMID:36403035). Proenkephalin (PENK) represents a new candidate to determine kidney function. This peptide is cleaved from the precursor peptide preproenkephalin A alongside enkephalins (endogenous opioids) and is filtrated in the glomerulus (PMID: 33636859).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Proenkephalin-A antibody 32272-1-AP | Download protocol |
| IHC protocol for Proenkephalin-A antibody 32272-1-AP | Download protocol |
| WB protocol for Proenkephalin-A antibody 32272-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Proenkephalin-A Polyclonal antibody (Cat# 32272-1-AP) has 1 total citation. It appears in Brain Research (Impact Factor 3.2), published in 2026. The cited study investigates duration-dependent effects of sucrose intake on fear extinction via distinct mechanisms in the amygdala and hippocampus, placing it in the neuroscience field, specifically related to neurobehavioral and opioid neuropeptide research.
| Species | Application | Title |
|---|---|---|
Brain Res Duration-dependent effects of sucrose intake on fear extinction via distinct mechanisms in the amygdala and hippocampus. |
