Tested Applications
| Positive WB detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32373-1-AP targets USP9Y in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35809 Product name: Recombinant human USP9Y protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2361-2555 aa of NM_004654 Sequence: KGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSSCPVAYQILQGNGDLKRKWTWAVEWLGDELERRPYTGNPQYSYNNWSPPVQSNETANGYFLERSHSARMTLAKACELCPEEEPDDQDAPDEHEPSPSEDAPLYPHSPASQYQQNNHVHGQPYTGPAAHHLNNPQKTGQRTQENYEGNEEVSSPQMKDQ Predict reactive species |
| Full Name | ubiquitin specific peptidase 9, Y-linked |
| Calculated Molecular Weight | 291kDa |
| Observed Molecular Weight | 250 kDa |
| GenBank Accession Number | NM_004654 |
| Gene Symbol | USP9Y |
| Gene ID (NCBI) | 8287 |
| RRID | AB_3742583 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O00507 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
USP9Y (Ubiquitin-Specific Peptidase 9 on the Y chromosome) is a gene located in the male-specific region of the Y chromosome and is broadly expressed in male adult tissues. It encodes a functional deubiquitinating enzyme that plays a role in protein turnover (PMID: 39742627).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for USP9Y antibody 32373-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this product is a 5-star rating. The reviewer used it at a 1:2000 dilution for Western Blot and cell culture, working with eukaryotic cells. They noted they were measuring USP9Y protein expression in human cancer cell lines. Overall, the feedback is positive, with no reported issues regarding performance or specificity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Karthik (Verified Customer) (04-22-2026) | We are going to measure the USP9Y protein expression with human cancer lines.
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