Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, Raji cells, Ramos cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32481-1-AP targets ZCCHC4 in WB, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34661 Product name: Recombinant human ZCCHC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-230 aa of BC157834 Sequence: MAASRNGFEAVEAEGSAGCRGSSGMEVVLPLDPAVPAPLCPHGPTLLFVKVTQGKEETRRFYACSACRDRKDCNFFQWEDEKLSGARLAAREAHNRRCQPPLSRTQCVERYLKFIELPLTQRKFCQTCQQLLLPDDWGQHSEHQVLGNVSITQLRRPSQLLYPLENKKTNAQYLFADRSCQFLVDLLSALGFRRVLCVGTPRLHELIKLTASGDKKSNIKSLLLDIDFRY Predict reactive species |
| Full Name | zinc finger, CCHC domain containing 4 |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC157834 |
| Gene Symbol | ZCCHC4 |
| Gene ID (NCBI) | 29063 |
| RRID | AB_3742627 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9H5U6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZCCHC4 antibody 32481-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZCCHC4 Polyclonal antibody (Cat# 32481-1-AP) has a total of 1 citation. It appears in Journal of Experimental & Clinical Cancer Research (IF: 14.3), published in 2024. The cited study investigates how the RNA m6A methylase ZCCHC4 mediates epigenetic downregulation of LncGHRLOS to promote colorectal cancer tumorigenesis, placing this citation in the research fields of epigenetics, RNA modification, and oncology.
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res The epigenetic downregulation of LncGHRLOS mediated by RNA m6A methylase ZCCHC4 promotes colorectal cancer tumorigenesis.
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