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Cyclin B1 Polyclonal antibody

Cyclin B1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Cat No. 55004-1-AP

Publications (360)

Reviews (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (5)

Applications

WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Synonyms

CCNB1, CCNB, Cyclin B, G2/mitotic specific cyclin B1, G2/mitotic-specific cyclin-B1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

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Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 55004-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 55004-1-AP
WB result of Cyclin B1 antibody (55004-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B1 transfected HeLa cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 55004-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 55004-1-AP
WB result of Cyclin B1 antibody (55004-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B1 transfected HeLa cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 55004-1-AP
Non-treated HeLa and thymidine or nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 55004-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of NIH/3T3 using 55004-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 55004-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 55004-1-AP
IP result of anti-Cyclin B1 (IP:55004-1-AP, 4ug; Detection:55004-1-AP 1:2000) with HeLa cells lysate 1560 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skin cancer using 55004-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 55004-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skin cancer using 55004-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 55004-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 55004-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin B1 antibody (55004-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of U2OS using 55004-1-AP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Cyclin B1 antibody (55004-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of HeLa using 55004-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.4 ug Cyclin B1 Polyclonal antibody (55004-1-AP)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (360)
Protocols
Reviews (2)

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, PC-3 cells, Jurkat cells, K-562 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, U2OS cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55004-1-AP targets Cyclin B1 in WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, rabbit, bovine, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	cyclin B1
Calculated Molecular Weight	48 kDa
Observed Molecular Weight	55-60 kDa
GenBank Accession Number	NM_031966
Gene Symbol	Cyclin B1
Gene ID (NCBI)	891
RRID	AB_10859790
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P14635
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

Protocols

Product Specific Protocols
FC protocol for Cyclin B1 antibody 55004-1-AP	Download protocol
IF protocol for Cyclin B1 antibody 55004-1-AP	Download protocol
IHC protocol for Cyclin B1 antibody 55004-1-AP	Download protocol
IP protocol for Cyclin B1 antibody 55004-1-AP	Download protocol
WB protocol for Cyclin B1 antibody 55004-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The anti-Cyclin B1 antibody (Cat# 55004-1-AP) has accumulated 360 citations. Publications appear in high-impact journals including Science, Nature Communications, Cell Death & Disease, Oncogene, Cell Reports, Phytomedicine, and International Journal of Oncology. Research fields span cell cycle regulation, mitosis, cancer biology, proliferation, apoptosis, ferroptosis, autophagy, drug discovery, and tissue regeneration, with applications predominantly in Western blot across human, mouse, rat, and bovine species.

Species	Application	Title
mouse	WB	Signal Transduct Target Ther MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration Authors - Jinpeng Du View Article
human	WB,IF	Science DNA-protein cross-links promote cGAS-STING-driven premature aging and embryonic lethality. Authors - Ines Tomaskovic View Article
human	WB	Mol Cancer Linc00659, a long noncoding RNA, acts as novel oncogene in regulating cancer cell growth in colorectal cancer. Authors - Kuo-Wang Tsai View Article
human	WB	Cancer Commun (Lond) CRISPR/Cas9 screening identifies a kinetochore-microtubule dependent mechanism for Aurora-A inhibitor resistance in breast cancer. Authors - Ailin Chen View Article
mouse	WB	Kidney Int Myeloid-derived Growth Factor Deficiency Exacerbates Mitotic Catastrophe of Podocytes in Glomerular Disease. Authors - Ping Zhan View Article
human	WB	Autophagy PRCC-TFE3 fusion-mediated PRKN/parkin-dependent mitophagy promotes cell survival and proliferation in PRCC-TFE3 translocation renal cell carcinoma. Authors - Bo Wang View Article

Reviews

5.0 out of 5

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2 verified customer reviews

What customers say

Both reviewers rated this antibody 5 stars for Western Blot applications. One user successfully detected Cyclin B1 in human U2OS cells at a 1:2000 dilution, describing it as a good antibody. Another user tested it on RKO cells at a 1:3000 dilution, calling it excellent. Overall, the reviews highlight reliable performance and strong detection in WB across different cell lines and dilutions.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Tsimafei (Verified Customer) (08-30-2025)	Detection of Cyclin B1 in human U2OS cells. Good antibody! Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: U2OS Cyclin B1 Antibody Western Blot validation (1:2000 dilution) in U2OS (Cat no:55004-1-AP)
Aleksandr (Verified Customer) (07-29-2025)	Excellent antibody Applications: Western Blot Primary Antibody Dilution: 1:3000 Cell Tissue Type: RKO Cyclin B1 Antibody Western Blot validation (1:3000 dilution) in RKO (Cat no:55004-1-AP)