Tested Applications
| Positive WB detected in | Jurkat cells, HEK-293 cells, mouse brain tissue |
| Positive IF/ICC detected in | HaCaT cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55063-1-AP targets SORCS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | sortilin-related VPS10 domain containing receptor 2 |
| Calculated Molecular Weight | 128 kDa |
| Observed Molecular Weight | 128-140 kDa |
| GenBank Accession Number | NM_020777 |
| Gene Symbol | SORCS2 |
| Gene ID (NCBI) | 57537 |
| RRID | AB_10859831 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96PQ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SORCS2, also named as KIAA1329, is one of the VPS10 domain-containing receptors. It contains 6 BNR repeats and 1 PKD domain.. The antibody is specific to SORCS2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SORCS2 antibody 55063-1-AP | Download protocol |
| WB protocol for SORCS2 antibody 55063-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SORCS2 Polyclonal antibody (Cat# 55063-1-AP) has 2 citations. One appears in PLoS One (2025), studying oxidative stress-responsive genes in recurrent miscarriage using IHC in human samples. The other is in Cytojournal (2025), investigating retinal ischemia-reperfusion-induced microglial activation via SORCS2/laminin upregulation using WB in rat. Research fields span reproductive pathology and neuroinflammation/retinal vascular biology.
| Species | Application | Title |
|---|---|---|
PLoS One Identification of oxidative stress-responsive genes in recurrent miscarriage and their role in disease pathogenesis. | ||
Cytojournal Astragalus polysaccharide attenuates retinal ischemia reperfusion-induced microglial activation through sortilin-related vacuolar protein sorting 10 domain containing receptor 2/laminin subunit alpha 1 upregulation |
Reviews
What customers say
The single available review is negative. The user attempted the antibody for both Western Blot and Immunohistochemistry on postnatal cerebellum tissue (P2–P14). They tested multiple lysis buffers and blocking conditions (5% milk, 2% BSA) at dilutions ranging from 1:100 to 1:1000, but reported that the antibody did not produce usable results in either application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Juan Pablo (Verified Customer) (11-29-2018) | I tried with different lysis buffer. I tried blocking with 5% milk or 2% BSA. Never worked for me. Didn't work for immunohistochemistry either dilution 1:1000 and 1:100.
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