Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue, SH-SY5Y cells, rat brain tissue |
| Positive IP detected in | SH-SY5Y cells |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF-Fro detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55084-1-AP targets DRD2 in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | dopamine receptor D2 |
| Calculated Molecular Weight | 51 kDa |
| Observed Molecular Weight | 50-60 kDa |
| GenBank Accession Number | NM_016574 |
| Gene Symbol | DRD2 |
| Gene ID (NCBI) | 1813 |
| RRID | AB_10859941 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14416 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DRD2, also named D2DR and D2R, belongs to the G-protein coupled receptor 1 family. It is one of the five types (D1 to D5) of receptors for dopamine. The activity of DRD2 is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11). The antibody is specific to DRD2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DRD2 antibody 55084-1-AP | Download protocol |
| IP protocol for DRD2 antibody 55084-1-AP | Download protocol |
| WB protocol for DRD2 antibody 55084-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DRD2 Polyclonal antibody (Cat# 55084-1-AP) has 66 citations across journals including Cell, Nature Communications, Molecular Psychiatry, Advanced Science, Molecular Neurodegeneration, Journal of Neuroinflammation, International Journal of Molecular Sciences, Journal of Ethnopharmacology, and Cancers. Research fields span dopamine signaling, Parkinson's disease, psychiatric disorders (depression, schizophrenia, ADHD), migraine and pain, addiction, cancer, and endocrinology (prolactin regulation), with applications mainly in WB, IF, and IHC across mouse, rat, and human.
| Species | Application | Title |
|---|---|---|
Adv Mater Intranasal Delivery of Near-infrared and Magnetic Dual-response Nanospheres to Rapidly Produce Antidepressant-like and Cognitive Enhancement Effects | ||
Mol Neurodegener Pathological characteristics of axons and alterations of proteomic and lipidomic profiles in midbrain dopaminergic neurodegeneration induced by WDR45-deficiency | ||
Nat Commun Non-canonical interplay between glutamatergic NMDA and dopamine receptors shapes synaptogenesis | ||
Adv Sci (Weinh) Neuroligin 1 Regulates Autistic-Like Repetitive Behavior through Modulating the Activity of Striatal D2 Receptor-Expressing Medium Spiny Neurons | ||
J Immunother Cancer Pharmacologic targeting of the dopamine D2 receptor impacts the efficacy of immune checkpoint blockade in melanoma. |
Reviews
What customers say
Customers report consistent performance across multiple applications. One user validated the antibody for immunofluorescence at 1/200 dilution, noting it worked very well for tagging validation. Another confirmed successful use in western blotting on human cancer cell lines. A third user found it effective for both immunoprecipitation and western blot at 1:500 dilution on rat brain tissue. Overall, reviewers rate the antibody favorably for WB and IP applications, with one IF user also expressing satisfaction and intent to reorder.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Alexandre (Verified Customer) (01-31-2026) | Asked for a validation of tagging, works very well! will probably order a bit more for extra analysis
|
ARUNASALAM (Verified Customer) (05-29-2025) | This antibody worked for western blotting.
|
Ping (Verified Customer) (06-04-2019) | This antibody is good for both immunoprecipitation and western blot.
|
