Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, Jurkat cells, MCF-7 cell, SKOV-3 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55213-1-AP targets ARHGEF4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | Rho guanine nucleotide exchange factor (GEF) 4 |
| Calculated Molecular Weight | 79 kDa |
| Observed Molecular Weight | 72 kDa |
| GenBank Accession Number | NM_015320 |
| Gene Symbol | ARHGEF4 |
| Gene ID (NCBI) | 50649 |
| RRID | AB_2721023 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NR80 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ARHGEF4, also named as KIAA1112 and Asef, acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC, ARHGEF4 may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion.(PMID: 10873612). This antibody is specific to ARHGEF4.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ARHGEF4 antibody 55213-1-AP | Download protocol |
| IP protocol for ARHGEF4 antibody 55213-1-AP | Download protocol |
| WB protocol for ARHGEF4 antibody 55213-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The APC antibody (Cat# 55213-1-AP) has 8 total citations. These appear in journals including Nature Communications, Nature Chemical Biology, International Journal of Oncology, iScience, Journal of Neuroscience, Cell Adhesion & Migration, Biology of Reproduction, and Oncotarget. Research fields span cancer biology (colorectal, pancreatic, and lung cancer), cell signaling and cytoskeletal organization, astrocyte development and injury response, and testicular Sertoli cell biology. Applications include WB, CoIP, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Nat Commun Rational design of a sensitivity-enhanced tracer for discovering efficient APC-Asef inhibitors | ||
Nat Chem Biol Peptidomimetic inhibitors of APC-Asef interaction block colorectal cancer migration. | ||
Int J Oncol ARHGEF4 predicts poor prognosis and promotes cell invasion by influencing ERK1/2 and GSK-3α/β signaling in pancreatic cancer. | ||
iScience Whole-genome DNA methylation and DNA methylation-based biomarkers in lung squamous cell carcinoma | ||
Cell Adh Migr ARHGEF4-mediates the actin cytoskeleton reorganization of hepatic stellate cells in 3-dimensional collagen matrices.
| ||
J Neurosci Temporal Profiling of Astrocyte Precursors Reveals Parallel Roles for Asef during Development and after Injury.
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