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What's the best dilution for BNC2 in ?
Tested Applications
| Positive WB detected in | HeLa cells, mouse uterus tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55220-1-AP targets BNC2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | basonuclin 2 |
| Calculated Molecular Weight | 122 kDa |
| Observed Molecular Weight | 122-140 kDa |
| GenBank Accession Number | NM_017637 |
| Gene Symbol | BNC2 |
| Gene ID (NCBI) | 54796 |
| RRID | AB_2881290 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZN30 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BNC2 antibody 55220-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-BNC2 antibody (Cat# 55220-1-AP) has 4 total citations across Nature Communications, Cell Death & Disease, Medical Oncology, and Advanced Science. Research fields include fibrosis (myofibroblastic activation), O-GlcNAcylation-mediated pro-fibrotic signaling, pancreatic cancer progression via EMT, and melanoma suppression through BNC2 degradation. Applications span WB, IP, and IHC in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Functional genomics uncovers the transcription factor BNC2 as required for myofibroblastic activation in fibrosis
| ||
Cell Death Dis O-GlcNAcylation controls pro-fibrotic transcriptional regulatory signaling in myofibroblasts | ||
Med Oncol BNC2 as a novel driver of pancreatic cancer progression through transcriptional regulation of COL3A1 and epithelial-to-mesenchymal transition. | ||
Adv Sci (Weinh) Natural Product Toosendanin Suppresses the Malignant Development of Skin Melanoma by Targeting BNC2 for Degradation.
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