Tested Applications
| Positive WB detected in | mouse kidney tissue |
| Positive IHC detected in | mouse bladder tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55229-1-AP targets UPK3A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | uroplakin 3A |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 31-33 kDa |
| GenBank Accession Number | NM_006953 |
| Gene Symbol | UPK3A |
| Gene ID (NCBI) | 7380 |
| RRID | AB_3670244 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75631 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin III (UPK3A) is a promising new urine marker for bladder cancer detection (PMID: 20346489). Loss of UPK3A expression is associated with clinicopathological features of invasive bladder cancer and poor prognosis in upper tract urothelial carcinoma (PMID: 18313120; PMID: 16686732). UPK3A has a calculated molecular weight of 30.7 kDa, with an apparent molecular weight of 47 kDa due to three potential N-glycosylation sites.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for UPK3A antibody 55229-1-AP | Download protocol |
| WB protocol for UPK3A antibody 55229-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
