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What's the best dilution for GPR55 in ?
Tested Applications
| Positive IHC detected in | human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55249-1-AP targets GPR55 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | G protein-coupled receptor 55 |
| Calculated Molecular Weight | 37 kDa |
| GenBank Accession Number | NM_005683 |
| Gene Symbol | GPR55 |
| Gene ID (NCBI) | 9290 |
| RRID | AB_2881294 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2T6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GPR55 antibody 55249-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GPR55 Polyclonal antibody (Cat# 55249-1-AP) has 2 total citations. These appear in Oxidative Medicine and Cell Longevity and Biochimica et Biophysica Acta – General Subjects. The associated research fields include mitochondrial DNA integrity in hepatoma cells and hepatocellular carcinoma signaling via the GPR55/TP53/MAPK axis. Both studies utilized the antibody for Western blotting, with one additionally employing immunohistochemistry in human samples.
| Species | Application | Title |
|---|---|---|
Oxid Med Cell Longev Knockout of Mpv17-Like Protein (M-LPH) Gene in Human Hepatoma Cells Results in Impairment of mtDNA Integrity through Reduction of TFAM, OGG1, and LIG3 at the Protein Levels. | ||
Biochim Biophys Acta Gen Subj Cannabidiol reverts the malignant phenotype of hepatocellular carcinoma cells via the GPR55/TP53/MAPK axis |
