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CASK Polyclonal antibody

CASK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55277-1-AP

Synonyms

CAGH39, CASK, CMG, FGS4, hCASK, LIN2, MICPCH, Protein lin 2 homolog, TNRC8



Tested Applications

Positive WB detected inmouse brain tissue, mouse liver tissue, A549 cells, rat brain tissue
Positive IHC detected inrat small intestine tissue, mouse kidney tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55277-1-AP targets CASK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name calcium/calmodulin-dependent serine protein kinase (MAGUK family)
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 100-110 kDa, 50-55 kDa
GenBank Accession NumberNM_003688
Gene Symbol CASK
Gene ID (NCBI) 8573
RRIDAB_10858487
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CASK, also named as LIN2, belongs to the MAGUK family. It is a multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. It contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. CASK binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. It may mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. Defects in CASK are the cause of mental retardation X-linked CASK-related (MRXCASK) . Defects in CASK are the cause of FG syndrome type 4 (FGS4). This antibody can recognize all the isoform of CASK.

Protocols

Product Specific Protocols
WB protocol for CASK antibody 55277-1-APDownload protocol
IHC protocol for CASK antibody 55277-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols