Tested Applications
| Positive WB detected in | mouse liver tissue, L02 cells |
| Positive IF/ICC detected in | L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55289-1-AP targets MMP21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | matrix metallopeptidase 21 |
| Calculated Molecular Weight | 65 kDa |
| Observed Molecular Weight | 67 kDa |
| GenBank Accession Number | NM_147191 |
| Gene Symbol | MMP21 |
| Gene ID (NCBI) | 118856 |
| RRID | AB_2881299 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N119 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MMP21 belongs to the peptidase M10A family. It may have an important and specific function in tumor progression and embryogenesis. It cleaves alpha-1-antitrypsin. The proenzyme without the signal peptide has a calculated molecular mass of 62 kD; following activation by furin, the processed enzyme has a calculated molecular mass of 49 kD(PMID:12617721),and MMP21 protein was detected in fetal neuroectoderm, in placenta, and in leukocytes(OMIM).This antibody is specific to MMP21.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MMP21 antibody 55289-1-AP | Download protocol |
| WB protocol for MMP21 antibody 55289-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MMP21 antibody (Cat# 55289-1-AP) has 3 total citations published in Nature Communications, Cancer Science, and Human Genomics. Research fields include Wnt signaling and cancer cell invasion via NF-κB-MMP21 pathway, MMP21-mediated metastasis and macrophage M2 polarization in hepatocellular carcinoma, and de novo disruptive MMP21 variants as genetic risk factors in heterotaxy. Applications cited include IF, WB, and IHC in human and mouse models.
| Species | Application | Title |
|---|---|---|
Nat Commun Wnt activation disturbs cell competition and causes diffuse invasion of transformed cells through NF-κB-MMP21 pathway | ||
Cancer Sci Matrix metalloproteinase-21 promotes metastasis via increasing the recruitment and M2 polarization of macrophages in HCC
| ||
Hum Genomics De novo disruptive heterozygous MMP21 variants are potential predisposing genetic risk factors in Chinese Han heterotaxy children |
