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What's the best dilution for MYO5A in ?
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55318-1-AP targets MYO5A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | myosin VA (heavy chain 12, myoxin) |
| Calculated Molecular Weight | 215 kDa |
| Observed Molecular Weight | 210-220 kDa |
| GenBank Accession Number | NM_000259 |
| Gene Symbol | MYO5A |
| Gene ID (NCBI) | 4644 |
| RRID | AB_2918356 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y4I1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MYO5A antibody 55318-1-AP | Download protocol |
| WB protocol for MYO5A antibody 55318-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYO5A Polyclonal antibody (catalog number 55318-1-AP) has 1 citation. It appears in the journal Human Cell, in a 2025 study on exosomal miR-145-5p impairing neoadjuvant chemotherapy by suppressing TFRC-mediated macrophage iron uptake and driving M2 polarization in esophageal cancer. The research field is cancer biology, specifically esophageal cancer and macrophage immunology, with the antibody applied in Western blot (WB) on human samples.
| Species | Application | Title |
|---|---|---|
Hum Cell Exosomal miR-145-5p impairs neoadjuvant chemotherapy by suppressing TFRC-mediated macrophage iron uptake and driving M2 polarization in esophageal cancer. |
