Tested Applications
| Positive WB detected in | COLO 320 cells, MCF-7 cells, PC-3 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55374-1-AP targets TRIM33 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | tripartite motif-containing 33 |
| Calculated Molecular Weight | 122 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | NM_015906 |
| Gene Symbol | TRIM33 |
| Gene ID (NCBI) | 51592 |
| RRID | AB_11183035 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UPN9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIM33, also named as KIAA1113, RFG7 and TIF1G, belongs to the TRIM/RBCC family. It acts as an E3 ubiquitin-protein ligase. TRIM33 promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. It may act as a transcriptional repressor. It inhibits the transcriptional response to TGF-beta/BMP signaling cascade. TRIM33 plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3, TRIM33 stimulates erythroid differentiation of hematopoietic stem/progenitor. Defects in TRIM33 are a cause of thyroid papillary carcinoma (TPC). This antibody is specific to TRIM33. The calculated molecular weight of TRIM33 is 122kDa, but the molecular weight of detected is about 140-150 kDa as the same as the result from the paper(PMID:25639486;26592194).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TRIM33 antibody 55374-1-AP | Download protocol |
| WB protocol for TRIM33 antibody 55374-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody (Cat# 55374-1-AP) has 8 total citations. It appears in journals including Nature Communications, Nature Chemical Biology, Cell Death & Disease, Molecular Therapy, Journal of Translational Medicine, Experimental Dermatology, and Technology in Cancer Research & Treatment. Research fields span SUMO biology, protein kinase activity, renal carcinoma, metabolic reprogramming, glioma stem cells, psoriasis, gastric cancer EMT, and acute myeloid leukemia.
| Species | Application | Title |
|---|---|---|
Nat Chem Biol Molecular recording of cellular protein kinase activity with chemical labeling
| ||
Cell Death Dis The deubiquitinase YOD1 suppresses tumor progression by stabilizing ZNF24 in clear cell renal carcinoma | ||
Mol Ther Lactate-induced miR-7-5p/TRIM33 reprograms metabolic flux to suppress tumor growth and viral reactivation.
| ||
J Transl Med DMC-BH derivative DMC-GF inhibits the growth of glioma stem cells by targeting the TRIM33/SLC25A1/mitochondrial oxidative phosphorylation pathway | ||
Exp Dermatol Nuclear translocation of PKM2 mediates keratinocyte metabolic reprogramming in psoriasis |
