Tested Applications
| Positive WB detected in | PC-3 cells, mouse spleen tissue, mouse thymus tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55397-1-AP targets DR3 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | tumor necrosis factor receptor superfamily, member 25 |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 52 kDa, 17-20 kDa |
| GenBank Accession Number | NM_003790 |
| Gene Symbol | DR3 |
| Gene ID (NCBI) | 8718 |
| RRID | AB_11183036 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q93038 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TNFRSF25, also named as APO3, DDR3, DR3, TNFRSF12, WSL, WSL1, LARD, TRAMP and AIR, is a receptor for TNFSF12/APO3L/TWEAK. TNFRSF25 interacts directly with the adapter TRADD. It mediates activation of NF-kappa-B and induces apoptosis. TNFRSF25 may play a role in regulating lymphocyte homeostasis. It is constitutively and highly expressed by CD4(+)FoxP3(+) Tregs. This antibody can recognize all the isoform of TNFRSF25.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DR3 antibody 55397-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DR3 Polyclonal antibody (Cat# 55397-1-AP) has 5 total citations. These appear in Br J Pharmacol, J Ethnopharmacol, Life Sci, J Cereb Blood Flow Metab, and Exp Biol Med (Maywood). The cited research spans ferroptosis in steatohepatitis, atherosclerosis and chemokine signaling, chronic colitis and dendritic cell activation, vascular lesions in hypertensive intracerebral hemorrhage, and apoptosis in ovarian cancer—covering fields of liver disease, cardiovascular biology, immunology, neurology, and oncology.
| Species | Application | Title |
|---|---|---|
Br J Pharmacol Lactylation of lysine396 in TNFRSF25 by lysine acetyltransferase 6B aggravates ferroptosis in metabolic dysfunction-associated steatohepatitis. | ||
J Ethnopharmacol Transcriptomic analysis reveals the critical role of Chemokine signaling in the anti-atherosclerosis effect of Xuefu Zhuyu Decoction | ||
J Cereb Blood Flow Metab The role and mechanism of lysyl hydroxylase 1 (LH1) in vascular lesions in hypertensive intracerebral hemorrhage. | ||
Exp Biol Med (Maywood) Silencing of FAM111B inhibits tumor growth and promotes apoptosis by decreasing AKT activity in ovarian cancer |
