Tested Applications
| Positive IHC detected in | rat skin tissue, human paracancerous tissue of skin, mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat skin tissue, mouse skin tissue |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55439-1-AP targets Loricrin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | loricrin |
| Calculated Molecular Weight | 26 kDa |
| GenBank Accession Number | NM_000427 |
| Gene Symbol | Loricrin |
| Gene ID (NCBI) | 4014 |
| RRID | AB_2881332 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23490 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Loricrin, also named LOR and LRN, is a major component of the cornified cell envelope (CE) expressed late in epidermal differentiation in the granular layers of normal skin. This antibody is specific to Loricrin.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Loricrin antibody 55439-1-AP | Download protocol |
| IHC protocol for Loricrin antibody 55439-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Loricrin Polyclonal antibody (Cat# 55439-1-AP) has accumulated 47 citations published in journals including Nature Aging, Cell Death & Differentiation, EMBO Molecular Medicine, Journal of Allergy and Clinical Immunology, Journal of Investigative Dermatology, Acta Biomaterialia, and Frontiers in Immunology. The associated research fields primarily cover dermatology (atopic dermatitis, psoriasis, skin barrier dysfunction), epidermal differentiation, keratinocyte biology, wound healing, skin aging, and immunology.
| Species | Application | Title |
|---|---|---|
Nat Aging Skin chronological aging drives age-related bone loss via secretion of cystatin-A | ||
Gut Microbes Gut-derived genistein from Parabacteroides distasonis alleviates psoriatic inflammation via CD200-mediated NF-κB inhibition in mice. | ||
J Nanobiotechnology Exosomes from human induced pluripotent stem cells-derived keratinocytes accelerate burn wound healing through miR-762 mediated promotion of keratinocytes and endothelial cells migration. | ||
Cell Death Differ ENKD1 promotes epidermal stratification by regulating spindle orientation in basal keratinocytes. | ||
J Allergy Clin Immunol Melatonin treatment increases skin microbiota-derived propionic acid to alleviate atopic dermatitis | ||
Phytomedicine Targeting mitochondrial dysfunction in atopic dermatitis with trilinolein: A triacylglycerol from the medicinal plant Cannabis fructus |
