Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55485-1-AP targets Rootletin in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | ciliary rootlet coiled-coil, rootletin |
| Calculated Molecular Weight | 229 kDa |
| Observed Molecular Weight | 228 kDa |
| GenBank Accession Number | NM_014675 |
| Gene Symbol | Rootletin |
| Gene ID (NCBI) | 9696 |
| RRID | AB_2935543 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5TZA2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Rootletin is predicted to enable kinesin binding activity and structural molecule activity. Involved in several processes, including centriole-centriole cohesion; positive regulation of cilium assembly; and positive regulation of protein localization to cilium.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Rootletin antibody 55485-1-AP | Download protocol |
| WB protocol for Rootletin antibody 55485-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Antibody 55485-1-AP has 1 total citation. It appears in bioRxiv (2026), in a study on KDM4A's role in maintaining basal body architecture and protecting against ciliary destabilization. The research field is ciliary biology and cell structure. The antibody was applied in IF and CoIP using human samples.
| Species | Application | Title |
|---|---|---|
bioRxiv Lysine Demethylase 4A (KDM4A) Maintains Basal Body Architecture and Protects Against Ciliary Destabilization. |
