Tested Applications
| Positive WB detected in | Jurkat cells, human spleen tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60005-1-Ig targets CD2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, sheep |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0423 Product name: Recombinant human CD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-209 aa of BC033583 Sequence: KEITNALETWGALGQDINLDIPSFQMSDDIDDIKWEKTSDKKKIAQFRKEKETFKEKDTYKLFKNGTLKIKHLKTDDQDIYKVSIYDTKGKNVLEKIFDLKIQERVSKPKISWTCINTTLTCEVMNGTDPELNLYQDGKHLKLSQRVITHKWTTSLSAKFKCTAGNKVSKESSVEPVSCPEKGLD Predict reactive species |
| Full Name | CD2 molecule |
| Calculated Molecular Weight | 351 aa, 39 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC033583 |
| Gene Symbol | CD2 |
| Gene ID (NCBI) | 914 |
| ENSEMBL Gene ID | ENSG00000116824 |
| RRID | AB_2881350 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P06729 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD2 antibody 60005-1-Ig | Download protocol |
| WB protocol for CD2 antibody 60005-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD2 Monoclonal antibody (3A10B2), catalog number 60005-1-Ig, has 3 total citations. These appear in Cell Research, Animals (Basel), and Experimental Hematology. The cited research spans RNA m6A modification and retrotransposition, canine malignant mammary tumor prognosis, and hematopoietic cell development from human induced pluripotent stem cells in fetal sheep models. Applications used include WB and IF.
| Species | Application | Title |
|---|---|---|
Cell Res RNA m6A modification orchestrates a LINE-1-host interaction that facilitates retrotransposition and contributes to long gene vulnerability. | ||
Animals (Basel) Serum Level of Tumor-Overexpressed AGR2 Is Significantly Associated with Unfavorable Prognosis of Canine Malignant Mammary Tumors. | ||
Exp Hematol Fetal sheep support the development of hematopoietic cells in vivo from human induced pluripotent stem cells |
