Tested Applications
| Positive WB detected in | A549 cells, Daudi cells, Jurkat cells, NK-92 cells, THP-1 cells, Ramos cells, Raji cells, TF-1 cells |
| Positive IF-P detected in | mouse spleen tissue |
| Positive IF/ICC detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60006-1-Ig targets CD38 in WB, IF/ICC, IF-P, ELISA, Cell treatment applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0487 Product name: Recombinant human CD38 protein Source: e coli.-derived, PKG Tag: GST Domain: 1-300 aa of BC007964 Sequence: MANCEFSPVSGDKPCCRLSRRAQLCLGVSILVLILVVVLAVVVPRWRQQWSGPGTTKRFPETVLARCVKYTEIHPEMRHVDCQSVWDAFKGAFISKHPCNITEEDYQPLMKLGTQTVPCNKILLWSRIKDLAHQFTQVQRDMFTLEDTLLGYLADDLTWCGEFNTSKINYQSCPDWRKDCSNNPVSVFWKTVSRRFAEAACDVVHVMLNGSRSKIFDKNSTFGSVEVHNLQPEKVQTLEAWVIHGGREDSRDLCQDPTIKELESIISKRNIQFSCKNIYRPDKFLQCVKNPEDSSCTSEI Predict reactive species |
| Full Name | CD38 molecule |
| Calculated Molecular Weight | 300 aa, 34 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC007964 |
| Gene Symbol | CD38 |
| Gene ID (NCBI) | 952 |
| ENSEMBL Gene ID | ENSG00000004468 |
| RRID | AB_716994 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P28907 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD38 antibody 60006-1-Ig | Download protocol |
| WB protocol for CD38 antibody 60006-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-CD38 antibody (catalog #60006-1-Ig) has 25 citations. These appear in journals including Cell Metabolism, Nature Immunology, Nature Aging, Neuron, EMBO Journal, Cell Reports, and Journal of Immunology. Research fields span NAD⁺ metabolism and aging, immunology (PD-1, macrophages, NK cells), cancer (breast, gastric, pancreatic, colorectal), and neurology (stroke, tauopathy, hearing loss). Applications are mainly WB and IF across human, mouse, rat, and pig samples.
| Species | Application | Title |
|---|---|---|
Cell Metab Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation | ||
Nat Immunol PD-1 blockade in subprimed CD8 cells induces dysfunctional PD-1+CD38hi cells and anti-PD-1 resistance.
| ||
Drug Resist Updat The combination of flaxseed lignans and PD-1/ PD-L1 inhibitor inhibits breast cancer growth via modulating gut microbiome and host immunity | ||
Reviews
What customers say
The single review for this CD38 antibody (used in Western Blot) gives it a 5-star rating. The reviewer tested CD38 expression in kidney tissue from control and cisplatin-induced kidney injury models, reporting that the antibody performed well with high specificity and detected the expected molecular size. Overall, the feedback is positive, highlighting reliable performance and specificity for the intended application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Komuraiah (Verified Customer) (07-16-2021) | CD38 expression that I tested in kidney tissue from control and cisplatin induced kidney injury model. This antibody is working great with high specificity with suggested molecular size.
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