Tested Applications
| Positive WB detected in | HeLa cells, Daudi cells, Ramos cells, THP-1 cells, Jurkat cells, Raji cells, K-562 cells |
| Positive IHC detected in | human tonsillitis tissue, human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60042-1-Ig targets CXCR4 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1528 Product name: Recombinant human CXCR4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 192-352 aa of BC020968 Sequence: NDLWVVVFQFQHIMVGLILPGIVILSCYCIIISKLSHSKGHQKRKALKTTVILILAFFACWLPYYIGISIDSFILLEIIKQGCEFENTVHKWISITEALAFFHCCLNPILYAFLGAKFKTSAQHALTSVSRGSSLKILSKGKRGGHSSVSTESESSSFHSS Predict reactive species |
| Full Name | chemokine (C-X-C motif) receptor 4 |
| Calculated Molecular Weight | 352 aa, 40 kDa |
| Observed Molecular Weight | 60~70 kDa |
| GenBank Accession Number | BC020968 |
| Gene Symbol | CXCR4 |
| Gene ID (NCBI) | 7852 |
| RRID | AB_2091809 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Caprylic acid/ammonium sulfate precipitation |
| UNIPROT ID | P61073 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some research, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CXCR4 antibody 60042-1-Ig | Download protocol |
| WB protocol for CXCR4 antibody 60042-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CXCR4 antibody (Cat# 60042-1-Ig) has 97 citations published across over 70 journals, including Nature, Nature Communications, Blood, Cell Stem Cell, Advanced Materials, Advanced Science, Cell Death & Disease, and Journal of Experimental Medicine. Research fields span oncology (tumor metastasis, microenvironment), immunology, stem cell biology, neurology, cardiology, fibrosis, inflammation, hematology, and nanomedicine-based drug delivery.
| Species | Application | Title |
|---|---|---|
Adv Mater Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment | ||
Adv Mater PerC B-Cells Activation via Thermogenetics-Based CXCL12 Generator for Intraperitoneal Immunity Against Metastatic Disseminated Tumor Cells | ||
Nat Aging Age-related liver endothelial zonation triggers steatohepatitis by inactivating pericentral endothelium-derived C-kit | ||
Cell Mol Immunol Neutrophils disrupt B-1a cell homeostasis by targeting Siglec-G to exacerbate sepsis | ||
Reviews
What customers say
Most users report satisfactory performance. Three out of four reviewers gave 4–5 stars, confirming the antibody works well for Western Blot (1:1000), Immunohistochemistry on liver tissue (1:100), and Immunofluorescence/Flow Cytometry (1:100). One reviewer reported a failed result at 1:500 dilution for Immunofluorescence, giving only 1 star. Overall, the antibody is generally considered reliable across multiple applications, though one user experienced a non-working result, possibly related to dilution or protocol differences.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Amy (Verified Customer) (02-06-2026) | Worked ok
|
Nicole (Verified Customer) (02-04-2026) | Anticorps fonctionnant bien
|
MALLIKARJUNA (Verified Customer) (11-07-2025) | WORKS GOOD FOR IHC
|
Rui (Verified Customer) (05-08-2025) | It was not working
|
