Tested Applications
| Positive WB detected in | A549 cells, HCT 116 cells, HEK-293 cells, HT-29 cells, hTERT-RPE1 cells, T-47D cells |
| Positive IHC detected in | mouse kidney tissue, human breast cancer tissue, human kidney tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60063-1-Ig targets Stanniocalcin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Reactivity | human, mouse, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0359 Product name: Recombinant human STC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 11-291 aa of BC000658 Sequence: TLALVLATFDPARGTDATNPPEGPQDRSSQQKGRLSLQNTAEIQHCLVNAGDVGCGVFECFENNSCEIRGLHGICMTFLHNAGKFDAQGKSFIKDALKCKAHALRHRFGCISRKCPAIREMVSQLQRECYLKHDLCAAAQENTRVIVEMIHFKDLLLHEPYVDLVNLLLTCGEEVKEAITHSVQVQCEQNWGSLCSILSFCTSAIQKPPTAPPERQPQVDRTKLSRAHHGEAGHHLPEPSSRETGRGAKGERGSKSHPNAHARGRVGGLGAQGPSGSSEWE Predict reactive species |
| Full Name | stanniocalcin 2 |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC000658 |
| Gene Symbol | Stanniocalcin 2 |
| Gene ID (NCBI) | 8614 |
| RRID | AB_2197544 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O76061 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Stanniocalcin (STC) is a family of secreted glycoprotein hormones that originally discovered in the corpuscles of Stannius, an endocrine gland of fish. STC1 and STC2, two homologues of STC family, are reported to involve in calcium and phosphate homeostasis. It is expressed in a wide variety of tissues such as kidney, spleen, heart, and pancreas. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC2 overexpression could promote tumor cell proliferation, invasion and metastasis in prostate cancer, ovarian cancer or neuroblastoma. STC2 is also vital for cytoprotective properties when exposed to ER stress and hypoxia.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Stanniocalcin 2 antibody 60063-1-Ig | Download protocol |
| IHC protocol for Stanniocalcin 2 antibody 60063-1-Ig | Download protocol |
| WB protocol for Stanniocalcin 2 antibody 60063-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Stanniocalcin 2 Monoclonal antibody (Cat# 60063-1-Ig) has 11 total citations. These appear in journals including Advanced Science, Cell Death & Disease, NPJ Precision Oncology, Journal of Hepatocellular Carcinoma, Archives of Biochemistry and Biophysics, Experimental Dermatology, Neuroscience, Biomedical Research International, BMC Medical Genomics, Biochemical and Biophysical Research Communications, and Oncotarget. The research spans oncology (laryngeal, lung, colorectal, liver, pancreatic, osteosarcoma, and head/neck cancers), fibrosis/keloid pathogenesis, and ischemic preconditioning, primarily using WB and IHC applications.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Smoking-Induced STC2(+) Tumor Cells Drive Tumor-Vascular Crosstalk in Laryngeal Squamous Cell Carcinoma via Spatial and Single-Cell Transcriptomics.
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Cell Death Dis A novel identified epithelial ligand-receptor-associated gene signature highlights POPDC3 as a potential therapy target for non-small cell lung cancer | ||
NPJ Precis Oncol FUT3 mediated GRP78 fucosylation promotes colorectal cancer survival and proliferation under glucose restriction via PERK/ATF4/STC2 axis. | ||
J Hepatocell Carcinoma The Oncogenic and Diagnostic Potential of Stanniocalcin 2 in Hepatocellular Carcinoma. | ||
Arch Biochem Biophys STC2 knockdown inhibits cell proliferation and glycolysis in hepatocellular carcinoma through promoting autophagy by PI3K/Akt/mTOR pathway | ||
Exp Dermatol STC2 Serves as a Critical Hypoxic Effector in Keloid Pathogenesis by Orchestrating Fibroblasts Activation and ECM Remodelling.
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