Tested Applications
| Positive WB detected in | Jurkat cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60077-1-Ig targets RAC2 in WB, IHC, IF, coIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1202 Product name: Recombinant human RAC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-192 aa of BC001485 Sequence: MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDSKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASYENVRAKWFPEVRHHCPSTPIILVGTKLDLRDDKDTIEKLKEKKLAPITYPQGLALAKEIDSVKYLECSALTQRGLKTVFDEAIRAVLCPQPTRQQKRACSLL Predict reactive species |
| Full Name | ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2) |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC001485 |
| Gene Symbol | RAC2 |
| Gene ID (NCBI) | 5880 |
| RRID | AB_2253445 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P15153 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAC2, also named as GX, belongs to the small GTPase superfamily and Rho family. RAC2 is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. RAC2 augments the production of reactive oxygen species (ROS) by NADPH oxidase. Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome. This target can exhibit cross-reactions with RAC1 and RAC3.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RAC2 antibody 60077-1-Ig | Download protocol |
| WB protocol for RAC2 antibody 60077-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RAC2 Monoclonal antibody (Cat# 60077-1-Ig) has 21 citations spanning journals including Cell Mol Immunol, ACS Nano, J Exp Med, Sci Signal, JCI Insight, Oncotarget, J Biol Chem, Development, Cancer Med, and Pathol Res Pract. Research fields covered include immunology (antifungal immunity, neutrophil mobilization), oncology (renal, prostate, nasopharyngeal, esophageal, rectal cancers), neuroscience (Alzheimer's, actin-mediated neurotransmission), inflammation, cardiology, nephrology, and ecotoxicology. Applications are predominantly WB, with some IHC, IF, and Co-IP.
| Species | Application | Title |
|---|---|---|
ACS Nano Oxidation of Reduced Graphene Oxide via Cellular Redox Signaling Modulates Actin-Mediated Neurotransmission. | ||
Cell Rep Med Macrophage efferocytosis mediated by the TP63-RAC2 pathway promotes immunosuppressive remodeling in esophageal cancer. | ||
J Exp Med Chemokine-coupled β2 integrin-induced macrophage Rac2-Myosin IIA interaction regulates VEGF-A mRNA stability and arteriogenesis.
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NPJ Sci Food Synaptic rescue in an Alzheimer's mouse model: low-temperature steam-derived black ginseng oligosaccharides remodel protein S-nitrosylation-NADPH oxidase axis. | ||
Int J Oncol RAC2 acts as a prognostic biomarker and promotes the progression of clear cell renal cell carcinoma.
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