Tested Applications
| Positive WB detected in | HUVEC cells, COLO 320 cells, bEnd.3 cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60170-1-Ig targets TEM1/CD248 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13334 Product name: Recombinant human CD248 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 399-687 aa of BC051340 Sequence: EPTQPPDFALAYRPSFPEDREPQIPYPEPTWPPPLSAPRVPYHSSVLSVTRPVVVSATHPTLPSAHQPPVIPATHPALSRDHQIPVIAANYPDLPSAYQPGILSVSHSAQPPAHQPPMISTKYPELFPAHQSPMFPDTRVAGTQTTTHLPGIPPNHAPLVTTLGAQLPPQAPDALVLRTQATQLPIIPTAQPSLTTTSRSPVSPAHQISVPAATQPAALPTLLPSQSPTNQTSPISPTHPHSKAPQIPREDGPSPKLALWLPSPAPTAAPTALGEAGLAEHSQRDDRWL Predict reactive species |
| Full Name | CD248 molecule, endosialin |
| Calculated Molecular Weight | 757 aa, 81 kDa |
| Observed Molecular Weight | 150-160 kDa |
| GenBank Accession Number | BC051340 |
| Gene Symbol | TEM1 |
| Gene ID (NCBI) | 57124 |
| RRID | AB_10694834 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9HCU0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TEM1 (Tumor endothelial marker 1), also named as CD248, Endosialin and CD164L1, is a C-type lectin-like domain (CTLD) containing type I transmembrane glycoprotein. It is now considered to be a highly selective marker for activated perivascular and stromal cells, detected in most cancers and at least some inflammatory disorders. CD248 plays a role in tumor angiogenesis. It is a potential diagnostic tool and therapeutic target of inflammatory and malignant disease. Two isoforms of human TEM1 exist. The calculated molecular weights of the two isoforms are 81 kDa and 46 kDa, respectively. Native TEM1 can be glycosylated, and the glycosylated form has a larger apparent molecular weight than 81 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TEM1/CD248 antibody 60170-1-Ig | Download protocol |
| WB protocol for TEM1/CD248 antibody 60170-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TEM1/CD248 Monoclonal antibody (Cat# 60170-1-Ig) has 15 citations spanning journals including Cancer Research, Nature Communications, Advanced Science, Nature Cardiovascular Research, EBioMedicine, PNAS, Oncotarget, and Scientific Reports. Research fields cover tumor angiogenesis, cardiac fibrosis and remodeling, insulin resistance, glioblastoma, soft tissue sarcoma, atherosclerosis, diabetic nephropathy, heart failure, and obesity. Applications include WB, IHC, IF, IP, and CoIP across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Cancer Res CD248 Regulates Wnt Signaling in Pericytes to Promote Angiogenesis and Tumor Growth in Lung Cancer | ||
Nat Commun Targeted therapies of inflammatory diseases with intracellularly gelated macrophages in mice and rats | ||
Adv Sci (Weinh) Understanding Glioblastoma Dynamics Using 3D Organoids and Engineered Extracellular Matrix. | ||
Nat Cardiovasc Res Dynamic molecular atlas of cardiac fibrosis at single-cell resolution shows CD248 in cardiac fibroblasts orchestrates interactions with immune cells
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EBioMedicine CD248 promotes insulin resistance by binding to the insulin receptor and dampening its insulin-induced autophosphorylation
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