Tested Applications
| Positive WB detected in | mouse kidney tissue, human kidney tissue, pig spleen tissue, U2OS cells, mouse colon tissue, pig pancreas tissue, A431 cells, human placenta tissue, pig kidney tissue |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60233-1-Ig targets MYH9 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Reactivity | human, mouse, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1612 Product name: Recombinant human MYH9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1741-1960 aa of BC011915 Sequence: EQGNTELINDRLKKANLQIDQINTDLNLERSHAQKNENARQQLERQNKELKVKLQEMEGTVKSKYKASITALEAKIAQLEEQLDNETKERQAACKQVRRTEKKLKDVLLQVDDERRNAEQYKDQADKASTRLKQLKRQLEEAEEEAQRANASRRKLQRELEDATETADAMNREVSSLKNKLRRGDLPFVVPRRMARKGAGDGSDEEVDGKADGAEAKPAE Predict reactive species |
| Full Name | myosin, heavy chain 9, non-muscle |
| Calculated Molecular Weight | 227 kDa |
| Observed Molecular Weight | 230 kDa, 160 kDa |
| GenBank Accession Number | BC011915 |
| Gene Symbol | MYH9 |
| Gene ID (NCBI) | 4627 |
| RRID | AB_2881357 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P35579 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYH9 antibody 60233-1-Ig | Download protocol |
| IHC protocol for MYH9 antibody 60233-1-Ig | Download protocol |
| WB protocol for MYH9 antibody 60233-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-MYH9 antibody (Cat# 60233-1-Ig) has accumulated 20 citations across 19 journals, including Cell Stem Cell, Nature Communications, Cell Reports, EMBO Reports, Oncogene, EBioMedicine, Cell Death & Differentiation, Theranostics, and Journal of Virology. Research fields span cancer biology (leukemia, lymphoma, colorectal and cervical cancer), cytoskeleton and mechanotransduction, innate immunity and antiviral responses, sepsis, vascular remodeling, and muscle development. Applications cited include WB, IF, IHC, CoIP, and IP.
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Mechano-oncogenic cytoskeletal remodeling drives leukemic transformation with mitochondrial vesicle-mediated STING activation | ||
Nat Commun Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking. | ||
Nat Commun RPSA-OLFM4 axis governs neutrophil migration against bacterial infection and sepsis. | ||
Theranostics Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis. | ||
Cell Death Differ Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-β-catenin-STAT3 signaling
| ||
Cell Death Dis Cancer-associated SF3B1 mutation suppresses DNA repair by disrupting the organization of nuclear actin network.
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