Tested Applications
| Positive WB detected in | human heart tissue, rat heart, mouse heart |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1900-1:7600 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60280-1-Ig targets FABP3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21483 Product name: Recombinant human FABP3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-133 aa of BC007021 Sequence: MVDAFLGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDILTLKTHSTFKNTEISFKLGVEFDETTADDRKVKSIVTLDGGKLVHLQKWDGQETTLVRELIDGKLILTLTHGTAVCTRTYEKEA Predict reactive species |
| Full Name | fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor) |
| Calculated Molecular Weight | 15 kDa |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | BC007021 |
| Gene Symbol | FABP3 |
| Gene ID (NCBI) | 2170 |
| RRID | AB_2881398 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P05413 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FABP3 (fatty-acid-binding protein 3), also known as heart-type FABP or mammary-derived growth inhibitor (MDGI), is a small 15-kDa cytoplasmic protein transporting fatty acids and other lipophilic substances from the cytoplasm to the nucleus. It is most ubiquitously expressed in heart and skeletal muscle.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FABP3 antibody 60280-1-Ig | Download protocol |
| IHC protocol for FABP3 antibody 60280-1-Ig | Download protocol |
| WB protocol for FABP3 antibody 60280-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FABP3 Monoclonal antibody (2B5C1), catalog number 60280-1-Ig, has 7 citations published in journals including Molecular Medicine, Molecular & Cellular Proteomics, Journal of Cellular and Molecular Medicine, Medicine & Science in Sports & Exercise, Frontiers in Oncology, bioRxiv, and Research Square. Research fields span cardiovascular disease (aortic dissection), TGF-β signaling and ubiquitin proteasome pathways, oesophageal cancer prognostic modeling, cardiometabolic risk, thyroid carcinoma fatty acid metabolism, and Alzheimer's disease metabolic regulation.
| Species | Application | Title |
|---|---|---|
Mol Med Calpain inhibition as a novel therapeutic strategy for aortic dissection with acute lower extremity ischemia | ||
Mol Cell Proteomics Quantitative ubiquitylome analysis reveals specificity of RNF111/Arkadia E3 ubiquitin ligase for its degradative substrates SKI and SKIL/SnoN in TGF-β signaling pathway | ||
J Cell Mol Med Four transcription profile-based models identify novel prognostic signatures in oesophageal cancer. | ||
Med Sci Sports Exerc Voluntary Wheel Running Reduces Cardiometabolic Risks in Female Offspring Exposed to Lifelong High-Fat, High-Sucrose Diet | ||
Reviews
What customers say
The single review for this antibody comes from a researcher who used it for Western Blot on NRVCMs at a 1:1000 dilution. They rated it 5 stars and stated they were very happy with the results. Overall, the limited feedback is positive, indicating satisfactory performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Marco (Verified Customer) (07-04-2024) | We are very happy with the results of this antibody
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