Tested Applications
| Positive WB detected in | human milk, HeLa cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60307-1-Ig targets FOLR1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19959 Product name: Recombinant human FOLR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 55-93 aa of BC002947 Sequence: EQCRPWRKNACCSTNTSQEAHKDVSYLYRFNWNHCGEMA Predict reactive species |
| Full Name | folate receptor 1 (adult) |
| Calculated Molecular Weight | 257 aa, 30 kDa |
| Observed Molecular Weight | 36-38 kDa |
| GenBank Accession Number | BC002947 |
| Gene Symbol | FOLR1 |
| Gene ID (NCBI) | 2348 |
| RRID | AB_2881421 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P15328 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Folate receptor 1 (FOLR1), also known as folate receptor alpha or adult folate-binding protein (FBP), is a 38-kDa glycoprotein belonging to the folate receptor family (PMID:15094207; 23528302). The receptor binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. FOLR1 expression is often limited to the apical surfaces of epithelium in the lung, kidney and choroid plexus but is differentially overexpressed in a variety of solid tumors such as ovarian cancer, non-small cell lung cancer, breast cancer, kidney cancer and high-grade osteosarcoma (PMID: 23528302; 23357463).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FOLR1 antibody 60307-1-Ig | Download protocol |
| WB protocol for FOLR1 antibody 60307-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FOLR1 Monoclonal antibody (2B4B7), catalog number 60307-1-Ig, has 8 total citations published in ACS Nano, Biomed Pharmacother, Biosensors (Basel), Cells, J Mol Diagn, Antibodies (Basel), Arch Pathol Lab Med, and Am J Nucl Med Mol Imaging. Research fields span tumor therapy (apoptosis-ferroptosis synergy), ovarian cancer, testicular germ cell tumors, breast cancer, non-small cell lung cancer, antibody-drug conjugates, circulating tumor cell isolation, extracellular vesicle-based cancer detection, and dual-receptor PET imaging.
| Species | Application | Title |
|---|---|---|
ACS Nano High-Performance Self-Cascade Pyrite Nanozymes for Apoptosis-Ferroptosis Synergistic Tumor Therapy. | ||
Biomed Pharmacother Therapeutic targeting of folate receptor alpha (FRα) by antibody-polymer-drug conjugate in testicular germ cell tumors. | ||
Biosensors (Basel) Folic Acid-Modified Fluorescent-Magnetic Nanoparticles for Efficient Isolation and Identification of Circulating Tumor Cells in Ovarian Cancer. | ||
Cells Mirvetuximab Soravtansine Induces Potent Cytotoxicity and Bystander Effect in Cisplatin-Resistant Germ Cell Tumor Cells | ||
J Mol Diagn Colocalization of cancer-associated biomarkers on single extracellular vesicles for early detection of cancer | ||
Antibodies (Basel) Novel Anti-FOLR1 Antibody-Drug Conjugate MORAb-202 in Breast Cancer and Non-Small Cell Lung Cancer Cells.
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