Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, MCF-7 cells, EGF treated A549 cells, UV treated HeLa cells, TNF alpha treated MCF-7 cells |
| Positive IF/ICC detected in | UV(1hour),100 nM Calyculin A(30 minutes)treated HeLa cells, HeLa cells |
| Positive FC (Intra) detected in | UV (1 hour) and 100 nM Calyculin A (30 minutes) HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.10 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60479-1-Ig targets Phospho-STAT3 (Ser727) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | signal transducer and activator of transcription 3 (acute-phase response factor) |
| Calculated Molecular Weight | 770 aa, 88 kDa |
| Observed Molecular Weight | 85-90 kDa |
| GenBank Accession Number | BC000627 |
| Gene Symbol | STAT3 |
| Gene ID (NCBI) | 6774 |
| RRID | AB_3670262 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P40763 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-STAT3 (Ser727) antibody 60479-1-Ig | Download protocol |
| WB protocol for Phospho-STAT3 (Ser727) antibody 60479-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Phospho-STAT3 (Ser727) Monoclonal antibody (2G11G11), catalog number 60479-1-Ig, has accumulated 43 citations published in journals including Redox Biology, Cancer Letters, Free Radical Biology and Medicine, Stem Cell Research & Therapy, Cell Signaling, Journal of Translational Medicine, Bioactive Materials, Advanced Materials, Phytomedicine, and ACS Omega. The associated research spans oncology (breast, colorectal, lung, gastric, glioma), inflammation, fibrosis, neurology, diabetes, bone biology, hepatology, and immunology.
| Species | Application | Title |
|---|---|---|
Bioact Mater Integrated apoptotic extracellular vesicle-recruitment peptide coating reprograms the diabetic bone microenvironment and orchestrates enhanced implant osseointegration. | ||
Redox Biol iPSC-derived cardiomyocytes and engineered heart tissues reveal suppressed JAK2/STAT3 signaling in LMNA-related emery-dreifuss muscular dystrophy | ||
Cell Rep Med Metabolically engineered probiotic OMVs as nanovaccine mediating sequential immunomodulation for chronic bone infection immunotherapy. | ||
Carbohydr Polym Structure-dependent effects of yeast- and Gleditsia-derived mannan oligosaccharides on dextran sodium sulfate-induced colitis in mice. | ||
Cancer Lett Membrane bionic supramolecular nanomaterials for co-delivered of ribociclib and PEG10 siRNA for targeted therapy in breast cancer. | ||
Cell Commun Signal Fibroblast-derived POSTN promotes colorectal cancer progression under high-fat diet by reprogramming fatty acid metabolism in tumor cell. |
