Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, NIH/3T3 cells, HSC-T6 cells, UV treated HEK-293 cells, UV treated NIH/3T3 cells, UV treated HSC-T6 cells, Anisomycin treated HeLa cells |
| Positive IHC detected in | Jurkat cells Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60666-1-Ig targets Phospho-JNK (Thr183/Tyr185) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | mitogen-activated protein kinase 8 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 42 kDa, 50 kDa |
| GenBank Accession Number | NM_138982 |
| Gene Symbol | JNK |
| Gene ID (NCBI) | 5599 |
| RRID | AB_3743229 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P45983 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. The antibody can detect endogenous levels of p46 and p54 SAPK/JNK when phosphorylated at Thr183 and Tyr185. It will also react with JNK singly phosphorylated at Thr183.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Phospho-JNK (Thr183/Tyr185) antibody 60666-1-Ig | Download protocol |
| WB protocol for Phospho-JNK (Thr183/Tyr185) antibody 60666-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Phospho-JNK (Thr183/Tyr185) Monoclonal antibody (Cat# 60666-1-Ig) has accumulated 36 citations across journals including Advanced Science, Cancer Letters, Cell Reports, Genes & Development, Free Radical Biology and Medicine, Journal of Ethnopharmacology, International Immunopharmacology, and Translational Medicine. Associated research fields encompass cancer biology, neuroinflammation and ischemic stroke, fibrosis, bone biology, oxidative stress, liver injury, wound healing, immune regulation, and MAPK/JNK signaling pathway studies, utilizing human, mouse, rat, and chicken models.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Activating the Osteoblastic USP26 Pathway Alleviates Multi-Organ Fibrosis by Decreasing Insulin Resistance. | ||
Cancer Lett Histone lactylation-derived LINC01127 promotes the self-renewal of glioblastoma stem cells via the cis-regulating the MAP4K4 to activate JNK pathway. | ||
Mater Today Bio Proanthocyanidin capsules remodel the ROS microenvironment via regulating MAPK signaling for accelerating diabetic wound healing | ||
Adv Healthc Mater Source-Dependent Mitochondrial Transplantation Drives Schwann Cell Bioenergetic Reprogramming and Peripheral Nerve Regeneration. | ||
J Transl Med Nicotine promotes cardiac fibrosis by regulating miR-125b-5p maturation via HNRNPA2B1-mediated METTL14-dependent m⁶A methylation: therapeutic reversal by cinacalcet HCl. | ||
Int J Biol Macromol Isolation, structural characterization and immunoregulatory activity of a novel acidic polysaccharide from Ganoderma sinense. |
