MLH1 Monoclonal antibody

MLH1 Monoclonal Antibody for IHC, IHC-Autostainer, ELISA

Cat No. 60822-2-Ig

Clone No. 2D5A2

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Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IHC-Autostainer, ELISA

Synonyms

2D5A2, COCA2, FCC2, hMLH1, HNPCC

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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-/ -

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Filter: AllIHC-AutostainerIHC

IHC staining of human appendicitis using 60822-2-Ig
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 60822-2-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 60822-2-Ig
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach cancer using 60822-2-Ig
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach cancer using 60822-2-Ig
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of Human tonsillitis using 60822-2-Ig
Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:2000 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of Human tonsillitis using 60822-2-Ig
Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:2000 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human appendicitis using 60822-2-Ig
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human rectal cancer using 60822-2-Ig
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 60822-2-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 60822-2-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human appendicitis using 60822-2-Ig
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human rectal cancer using 60822-2-Ig
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colorectal cancer using 60822-2-Ig
Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 60822-2-Ig
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 60822-2-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human appendicitis using 60822-2-Ig
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach cancer using 60822-2-Ig
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach cancer using 60822-2-Ig
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60822-2-Ig (MLH1 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for MLH1 in ?

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive IHC-Autostainer detected in	human ovary cancer tissue, human stomach cancer tissue, human tonsillitis tissue, human appendicitis tissue
Positive IHC detected in	Human tonsillitis tissue, human stomach cancer tissue, human ovary cancer tissue, human colorectal cancer tissue, human rectal cancer tissue, human appendicitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)-AUTOSTAINER	IHC-AUTOSTAINER : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60822-2-Ig targets MLH1 in IHC, IHC-Autostainer, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag27723 Product name: Recombinant human MLH1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 407-756 aa of BC006850 Sequence: QPQAIVTEDKTDISSGRARQQDEEMLELPAPAEVAAKNQSLEGDTTKGTSEMSEKRGPTSSNPRKRHREDSDVEMVEDDSRKEMTAACTPRRRIINLTSVLSLQEEINEQGHEVLREMLHNHSFVGCVNPQWALAQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPIFILRLATEVNWDEEKECFESLSKECAMFYSIRKQYISEESTLSGQQSEVPGSIPNSWKWTVEHIVYKALRSHILPPKHFTEDGNILQLANLPDLYKVFERC Predict reactive species
Full Name	mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
Calculated Molecular Weight	756 aa, 85 kDa
GenBank Accession Number	BC006850
Gene Symbol	MLH1
Gene ID (NCBI)	4292
RRID	AB_3743234
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P40692
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer.