Tested Applications
| Positive WB detected in | LoVo cells, HSC-T6 cells, NIH/3T3 cells, A431 cells, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60903-1-Ig targets SAV1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3441 Product name: Recombinant human SAV1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-376 aa of BC020537 Sequence: MLSRKKTKNEVSKPAEVQGKYVKKETSPLLRNLMPSFIRHGPTIPRRTDICLPDSSPNAFSTSGDVVSRNQSFLRTPIQRTPHEIMRRESNRLSAPSYLARSLADVPREYGSSQSFVTEVSFAVENGDSGSRYYYSDNFFDGQRKRPLGDRAHEDYRYYEYNHDLFQRMPQNQGRHASGIGRVAATSLGNLTNHGSEDLPLPPGWSVDWTMRGRKYYIDHNTNTTHWSHPLEREGLPPGWERVESSEFGTYYVDHTNKKAQYRHPCAPSVPRYDQPPPVTYQPQQTERNQSLLVPANPYHTAEIPDWLQVYARAPVKYDHILKWELFQLADLDTYQGMLKLLFMKELEQIVKMYEAYRQALLTELENRKQRQQWYA Predict reactive species |
| Full Name | salvador homolog 1 (Drosophila) |
| Calculated Molecular Weight | 383 aa, 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC020537 |
| Gene Symbol | SAV1 |
| Gene ID (NCBI) | 60485 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9H4B6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The Salvador family WW domain‐containing 1 (SAV1, also known as WW45) is a human homologue of Salvador that contains two protein‐protein interaction modules known as WW domains and acts as a scaffolding protein (PMID: 28618450). SAV1 belongs to the core kinase components of Hippo signalling pathway for efficient MST1/2 activation. SAV1 represses the growth of colorectal cancer cells by inactivating Akt‐mTOR signalling in a YAP‐dependent manner (PMID: 29063833, 22185343).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SAV1 antibody 60903-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
