Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, HUVEC cells, COLO 320 cells, HepG2 cells, MOLT-4 cells, HSC-T6 cells, RAW 264.7 cells |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60929-1-Ig targets ERK1/2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | mitogen-activated protein kinase 1 |
| Calculated Molecular Weight | 360 aa, 41 kDa |
| Observed Molecular Weight | 38-44 kDa |
| GenBank Accession Number | BC017832 |
| Gene Symbol | ERK2 |
| Gene ID (NCBI) | 5594 |
| RRID | AB_3743244 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P28482 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ERK1 and ERK2 belong to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalyzed the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with a molecular mass of 38-44 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ERK1/2 antibody 60929-1-Ig | Download protocol |
| WB protocol for ERK1/2 antibody 60929-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
ERK1/2 Monoclonal antibody (1A4H10), catalog number 60929-1-Ig, has 3 total citations. These appear in Journal of Controlled Release, International Journal of Biological Macromolecules, and Journal of Cellular and Molecular Medicine. The associated research fields include ocular neovascular disease and drug delivery, adenoid cystic carcinoma and RNA modification signaling, and ischemic stroke-induced angiogenesis and neurogenesis. All citations used the antibody for Western blot in human or mouse samples.
| Species | Application | Title |
|---|---|---|
J Control Release Non-invasive co-delivery of Axitinib and telmisartan via LDH-based nanocarriers for synergistic VEGF/Ang-2 inhibition in ocular neovascular diseases. | ||
Int J Biol Macromol MYB-driven NAT10-IGF2BP3 RNA modification axis sustains MAPK signaling and promotes adenoid cystic carcinoma progression. | ||
J Cell Mol Med HIF-1α/Netrin-4 Axis Mediates RIPC-Induced Angiogenesis and Neurogenesis After Ischemic Stroke. |
