Tested Applications
| Positive FC detected in | mouse bone marrow cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65055-1-Ig targets CD11b in IF, FC applications and shows reactivity with human, mouse, rabbit, cynomolgus monkey, guinea pig samples.
| Tested Reactivity | human, mouse, rabbit, cynomolgus monkey, guinea pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes Predict reactive species |
| Full Name | integrin alpha M |
| Calculated Molecular Weight | 127 kDa |
| GenBank Accession Number | BC156991 |
| Gene Symbol | CD11b |
| Gene ID (NCBI) | 16409 |
| RRID | AB_2918363 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | E9Q604 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD11b antibody 65055-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Mouse CD11b (M1/70), catalog number 65055-1-Ig, has 16 citations published in journals including J Nanobiotechnology, Cell Reports in Medicine, Journal of Controlled Release, Cancer Letters, Advanced Healthcare Materials, Materials Today Bio, European Journal of Pharmacology, and others. Research fields span nanomedicine and drug delivery, cancer immunotherapy, neuroinflammation, ferroptosis, macrophage biology, and metabolic disease.
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology Extracellular vesicles derived from Kaempferia Galanga L. show promise for targeted oral therapy in the treatment of ulcerative colitis. | ||
Cell Rep Med Metabolically engineered probiotic OMVs as nanovaccine mediating sequential immunomodulation for chronic bone infection immunotherapy. | ||
J Control Release Unsaturated fatty acid-doped liposomes deliver piperine to deactivate defensive mechanism for ferroptosis in cancer therapy. | ||
Cancer Lett BMP9 potentiates immunotherapy in triple-negative breast cancer by suppressing Tregs infiltration via the PRKDC-CCL2 axis. | ||
Phytomedicine Daphnetin alleviates influenza pneumonia by enhancing chaperone-mediated autophagy of KEAP1 through Heat shock cognate 71 kDa protein. | ||
Adv Healthc Mater Glutathione-Depletable Nanoinducer for Boosting Immunomodulatory Synergistic Therapy to Reverse Breast Cancer Chemoresistance |
