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Sandwich ELISA standard curve of MP50064-1
Sandwich ELISA standard curve of MP50064-1, CD40 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66965-2-PBS. Detection antibody: 65103-1-PBS. Standard: Ag28040. Range: 78.1-5000 pg/mL.
Sample test of MP50064-1
Urine of eight individual healthy human donors was measured. The CD40 concentration of detected samples was determined to be 1,053.4 pg/mL with a range of 79.9-2,169.7 pg/mL.
Sample test of MP50064-1
Saliva of six individual healthy human donors was measured. The CD40 concentration of detected samples was determined to be 684.6 pg/mL with a range of 349.9-1,498.5 pg/mL.
Sample test of MP50064-1
Human milk of six individual healthy human donors was measured. The CD40 concentration of detected samples was determined to be 53.4 pg/mL with a range of 29.8-104.3 pg/mL.
Sample test of MP50064-1
Plasma of fifteen individual healthy human donors was measured. The CD40 concentration of detected samples was determined to be 89.6 pg/mL with a range of 39.1-155.7 pg/mL.
FC experiment of human PBMCs using 65103-1-Ig (same clone as 65103-1-PBS)
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD40 (65103-1-Ig, Clone:G28.5) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CoraLite®488 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65103-1-PBS in a different storage buffer formulation.
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1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD40 (65103-1-Ig, Clone:G28.5) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CoraLite®488 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65103-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65103-1-Ig (same clone as 65103-1-PBS)
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD40 (65103-1-Ig, Clone:G28.5) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65103-1-PBS in a different storage buffer formulation.

Anti-Human CD40 (G28.5), PBS Only (Detector)

CD40 Monoclonal Antibody for FC, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 65103-1-PBS

Clone No.G28.5

Review Product

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

B cell surface antigen CD40, B-cell surface antigen CD40, Bp50, CD40L receptor, G28.5

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

65103-1-PBS targets CD40 as part of a matched antibody pair:

MP50064-1: 66965-2-PBS capture and 65103-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Immunogen	N/A Predict reactive species
Full Name	CD40 molecule, TNF receptor superfamily member 5
Calculated Molecular Weight	277 aa, 31 kDa
GenBank Accession Number	BC012419
Gene Symbol	CD40
Gene ID (NCBI)	958
ENSEMBL Gene ID	ENSG00000101017
RRID	AB_2881471
Conjugate	Unconjugated
Form	Liquid
Purification Method	Affinity purification
UNIPROT ID	P25942
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).