Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65143-1-Ig targets CD4 in IF, FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | CD4 molecule |
| Calculated Molecular Weight | 51 kDa |
| GenBank Accession Number | BC025782 |
| Gene Symbol | CD4 |
| Gene ID (NCBI) | 920 |
| ENSEMBL Gene ID | ENSG00000010610 |
| RRID | AB_2918429 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P01730 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD4 antibody 65143-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Human CD4 (RPA-T4), catalog number 65143-1-Ig, has 3 citations. They appear in Materials Today Bio, Allergy Asthma Immunology Research, and Molecular Carcinogenesis. The research fields span prostate cancer therapy (tumor-resident bacteria-mediated treatment), allergic rhinitis (TET2-regulated 5-hydroxymethylcytosine and CD4⁺ T-cell balance), and melanoma (POU4F1-mediated resistance to anti-PD-1 immunotherapy via m7G methylation of PKM2). Applications cited include flow cytometry and immunofluorescence in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Mater Today Bio 3-acetyl-11-keto-β-boswellic acid and chitosan-Ag nanoparticles for synergistic tumor-resident bacteria mediated prostate cancer therapy. | ||
Allergy Asthma Immunol Res TET2 Regulates 5-Hydroxymethylcytosine Signature and CD4+ T-Cell Balance in Allergic Rhinitis. | ||
Mol Carcinog POU4F1 Promotes the Primary Resistance of Melanoma to Anti-PD-1 Therapy by Regulating Glycolysis Through METTL1-Mediated m7G Methylation of PKM2. |
Reviews
What customers say
All three reviews rated this product 5 stars. Users reported consistent performance in flow cytometry and immunofluorescence applications. The antibody worked well for conjugating custom fluorophores and dyes, and performed reliably with compensation beads for flow cytometry analysis. One user noted it functioned as expected for their custom dye conjugation workflow. Overall, reviewers found the product dependable and effective for their intended applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sunil (Verified Customer) (08-03-2023) | worked as expected for conjugating our custom dyes to
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Sareena (Verified Customer) (06-02-2023) | Worked well with compensation beads
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Sareena (Verified Customer) (04-20-2023) | Worked well with conjugating fluorophores and tested with compensation beads for flow cytometry analysis.
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